eISSN: 2353-9461
ISSN: 0860-7796
BioTechnologia
Current issue Archive About the journal Editorial board Abstracting and indexing Subscription Contact Instructions for authors Publication charge Ethical standards and procedures
Editorial System
Submit your Manuscript
3/2012
vol. 93
 
Share:
Share:
abstract:

RESEARCH PAPER
Structural changes of corn starch during fuel ethanol production from corn flour

Daria Szymanowska-Powałowska
,
Grażyna Lewandowicz
,
Wioletta Błaszczak
,
Artur Szwengiel

BioTechnologia vol. 93(3) C pp. 333-341 C 2012
Online publish date: 2014/10/29
View full text Get citation
 
PlumX metrics:
The key factor in production of fuel ethanol by simultaneous saccharification and fermentation is the efficient conversion

of granular starch into ethanol. Most difficult stage in the process is the enzymatic hydrolysis of starch

granules. Their supramolecular structure as well as crystallinity and presence of complexing agents are key factors

for the hydrolysis process. The aim of the study was to examine structural changes in starch granules during

the simultaneous processes of saccharification and fermentation of corn flour in the long-term repeated Simultaneous

Saccharification and Fermentation (SSF) process with complete recycling of the stillage liquid fraction. The

SSF experiments were performed using corn flour as a raw material, the STARGEN 001 preparation as a hydrolytic

enzyme, and Red Star Ethanol Red (Saccharomyces cerevisiae ) fermentation yeasts. Residual starch structure

after the 4th, and the 7th operation cycle was examined using scanning electron microscopy, X-ray diffractometry,

IR spectroscopy as well as gel permeation chromatography. In spite of accumulating glycerol, organic acids

and inorganic ions in the fermentation broth, the repeated batch SSF process, with stillage recycling into the fermentation

phase conducted on corn flour with the use of the STARGEN 001 enzyme preparation, was found to

run efficiently. The amount of unhydrolyzed residual starch was independent of the number of operation cycles.

Hydrolysis of starch resulted in the formation of porous granules and a small amount of undigested granules and

pyramid-shaped residuals. Crystalline and amorphous regions were evenly digested. The molecular mass distribution

of residual starch after the SSF process significantly differed from that of native starch both in the region

corresponding to amylopectin and to amylose, while the most distinctive changes with respect to the amylopectin/

amylose ratio, i.e. in the 4th cycle the amylopectin content decreased by up to 19%.
keywords:

ethanol, simultaneous saccharification and fermentation, native corn starch

 
Stosujemy się do standardu HONcode dla wiarygodnej informacji zdrowotnej This site complies with the HONcode standard for trustworthy health information: verify here