Journal of Plant Science

ISSN: 2573-7988

Impact Factor: 0.583

VOLUME: 2 ISSUE: 2

Page No: 65-72

Effects of Ganoderma Lucidum on Biochemical Dysfunctions of the Rabbit Urinary Bladder using an In-Vitro Model of Ischemia / Reperfusion


Co-Authors

*Robert M. Levin PhD, Li-Xia, Wu Wei, Catherine Schuler, Robert E. Leggett, Alpha D-Y Lin MD

Article Reviewed By:

Ulrike Lindequist(lindequi@uni-greifswald.de)

SIVAKUMAR MANICKAM(Sivakumar.Manickam@nottingham.edu.my)

Citation

Robert M. Levin PhD, Li-Xia, Wu Wei, Catherine Schuler, Robert E. Leggett, Alpha D-Y Lin MD, Effects of Ganoderma Lucidum on Biochemical Dysfunctions of the Rabbit Urinary Bladder using an In-Vitro Model of Ischemia / Reperfusion(2017)SDRP Journal of Plant Science 2(2):65-72

Abstract

Background: Oxidative stress involving ischemia followed by reperfusion are major etiological factors in obstructive bladder dysfunction in both men and rabbits. Specific natural products such as Ganoderma lucidum with significant antioxidant activity have proven to be useful in their treatment. In two recent studies we demonstrated that pretreatment with Ganoderma was effective preventing bladder dysfunction using both in-vivo and in-vitro models of ischemia-reperfusion. The current study is a follow-up study using the in-vitro model of ischemia-reperfusion.

Methods: Eight New Zealand White rabbits were divided into 2 groups. One group (Group-1) was fed Ganoderma (100 mg/Kg) daily for 3 weeks while the other group (Group-2) were given saline. At the end of a 3 week period, each rabbit was euthanized and the bladder separated into twelve full thickness strips and mounted in individual baths. After 1 hour in oxygenated Tyrodes with glucose, 4 strips from each group were removed frozen and stored at -80oC. At this time, the oxygenated Tyrodes + glucose were changed to Tyrodes equilibrated with nitrogen in the absence of glucose (ischemia) for 1 hour. After 1 hour of ischemia the buffer was then changed back to normal oxygenated Tyrode’s with glucose and allowed to recover for 2 hours (reperfusion). After this time period, the four remaining strips from each group were frozen and stored. The isolated strips were analyzed for the following biomarker enzymes: citrate synthase (mitochondria), calcium ATPase (intracellular calcium movement through the cell wall), and sarco-endoplasmic reticular ATPase (intracellular calcium uptake into the sarcoplasmic reticulum following stimulation.

Results: Ischemia-reperfusion resulted in a significant decrease (~50%) in all three enzyme activities. Pretreating with Ganoderma protected all three enzyme activities which were approximately at control levels.

Conclusion: Pretreatment of rabbits for three weeks with Ganoderma prior to subjecting them to in-vitro ischemia-reperfusion significantly protected the bladder enzymes.

References

  1. Sovari, A.A., Cellular and Molecular Mechanisms of Arrhythmia by Oxidative Stress. Cardiol Res Pract, 2016. 2016: p. 9656078. PMid:26981310

    PubMed/NCBI     
  2. Dhalla, N.S., et al., Evidence for the role of oxidative stress in acute ischemic heart disease: a brief review. Can J Cardiol, 1999. 15(5): p. 587-93. PMid:10350670

    PubMed/NCBI     
  3. Reddy, S. and D. Bernstein, Molecular Mechanisms of Right Ventricular Failure. Circulation, 2015. 132(18): p. 1734-42. PMid:26527692

    View Article      PubMed/NCBI     
  4. Nikooyeh, B. and T.R. Neyestani, Oxidative stress, type 2 diabetes and vitamin D: past, present and future. Diabetes Metab Res Rev, 2016. 32(3): p. 260-7. PMid:26409185

    View Article      PubMed/NCBI     
  5. Rani, V., et al., Oxidative stress and metabolic disorders: Pathogenesis and therapeutic strategies. Life Sci, 2016. 148: p. 183-93. PMid:26851532

    View Article      PubMed/NCBI     
  6. Stone, J.R. and L.R. Wilkins, Acute mesenteric ischemia. Tech Vasc Interv Radiol, 2015. 18(1): p. 24-30. PMid:25814200

    View Article      PubMed/NCBI     
  7. Stoney, R.J. and C.G. Cunningham, Acute mesenteric ischemia. Surgery, 1993. 114(3): p. 489-90. PMid:8367801

    PubMed/NCBI     
  8. Bhattacharyya, A., et al., Oxidative stress: an essential factor in the pathogenesis of gastrointestinal mucosal diseases. Physiol Rev, 2014. 94(2): p. 329-54. PMid:24692350

    View Article      PubMed/NCBI     
  9. Nastos, C., et al., Global consequences of liver ischemia/reperfusion injury. Oxid Med Cell Longev, 2014. 2014: p. 906965. PMid:24799983 PMCid:PMC3995148

    PubMed/NCBI     
  10. Tucker, P.S., A.T. Scanlan, and V.J. Dalbo, Chronic kidney disease influences multiple systems: describing the relationship between oxidative stress, inflammation, kidney damage, and concomitant disease. Oxid Med Cell Longev, 2015. 2015: p. 806358. PMid:25861414

    PubMed/NCBI     
  11. Tamma, G. and G. Valenti, Evaluating the Oxidative Stress in Renal Diseases: What Is the Role for S-Glutathionylation? Antioxid Redox Signal, 2016. PMid:26972776

    View Article      PubMed/NCBI     
  12. Juan, Y.S., et al., The effect of partial bladder outlet obstruction on carbonyl and nitrotyrosine distribution in rabbit bladder. Urology, 2007. 70(6): p. 1249-53. PMid:18158070

    View Article      PubMed/NCBI     
  13. Li, H.T., et al., Differential effects of coenzyme Q10 and alpha-lipoic acid on two models of in vitro oxidative damage to the rabbit urinary bladder. Int Urol Nephrol, 2011. 43(1): p. 91-7. PMid:20533089

    View Article      PubMed/NCBI     
  14. Nomiya, M., K.E. Andersson, and O. Yamaguchi, Chronic bladder ischemia and oxidative stress: new pharmacotherapeutic targets for lower urinary tract symptoms. Int J Urol, 2015. 22(1): p. 40-6. PMid:25339506

    View Article      PubMed/NCBI     
  15. Levin, R.M., et al., Partial outlet obstruction in rabbits: duration versus severity. Int J Urol, 2013. 20(1): p. 107-14. PMid:23050656

    View Article      PubMed/NCBI     
  16. Juan, Y.S., et al., The beneficial effect of coenzyme Q10 and lipoic acid on obstructive bladder dysfunction in the rabbit. J Urol, 2008. 180(5): p. 2234-40. PMid:18804800

    View Article      PubMed/NCBI     
  17. Lindblom, R., et al., Targeting Mitochondria and Reactive Oxygen Species-Driven Pathogenesis in Diabetic Nephropathy. Rev Diabet Stud, 2015. 12(1-2): p. 134-56. PMid:26676666

    View Article      PubMed/NCBI     
  18. Saha, S.P. and T.F. Whayne, Jr., Coenzyme Q-10 in Human Health: Supporting Evidence? South Med J, 2016. 109(1): p. 17-21. PMid:26741866

    View Article      PubMed/NCBI     
  19. Radu, F., et al., The effect of antioxidants on the response of the rabbit urinary bladder to in vitro ischemia/reperfusion. Mol Cell Biochem, 2011. 355(1-2): p. 65-73. PMid:21541678

    View Article      PubMed/NCBI     
  20. Kim, J., et al., Phytochemicals in Ischemic Stroke. Neuromolecular Med, 2016. PMid:27193940

    View Article      PubMed/NCBI     
  21. Xie, C., et al., Persimmon (Diospyros kaki L.) leaves: a review on traditional uses, phytochemistry and pharmacological properties. J Ethnopharmacol, 2015. 163: p. 229-40. PMid:25637828

    View Article      PubMed/NCBI     
  22. Yu, L., et al., The efficacy and safety of Chinese herbal medicine, Rhodiola formulation in treating ischemic heart disease: a systematic review and meta-analysis of randomized controlled trials. Complement Ther Med, 2014. 22(4): p. 814-25. PMid:25146085

    View Article      PubMed/NCBI     
  23. Juan, Y.S., et al., Protective effect of Antrodia camphorata on bladder ischemia/reperfusion injury. Int Urol Nephrol, 2010. 42(3): p. 637-45. PMid:19760512

    View Article      PubMed/NCBI     
  24. Levin, R.M., et al., Kohki tea protects the rabbit bladder from ischemia/reperfusion-induced contractile dysfunction. Urol Int, 2008. 80(4): p. 425-30. PMid:18587255

    View Article      PubMed/NCBI     
  25. Francis, J.A., et al., Comparative biochemical responses and antioxidant activities of the rabbit urinary bladder to whole grapes versus resveratrol. Mol Cell Biochem, 2015. 410(1-2): p. 121-9. PMid:26354548

    View Article      PubMed/NCBI     
  26. Francis, J.A., et al., Effect of hydrogen peroxide on contractility and citrate synthase activity of the rabbit urinary bladder in the presence and absence of resveratrol and a whole-grape suspension. Mol Cell Biochem, 2014. 391(1-2): p. 233-9. PMid:24627242

    View Article      PubMed/NCBI     
  27. Suksomboon, N., N. Poolsup, and N. Juanak, Effects of coenzyme Q10 supplementation on metabolic profile in diabetes: a systematic review and meta-analysis. J Clin Pharm Ther, 2015. 40(4): p. 413-8. PMid:25913756

    View Article      PubMed/NCBI     
  28. Ozkanlar, S. and F. Akcay, Antioxidant vitamins in atherosclerosis--animal experiments and clinical studies. Adv Clin Exp Med, 2012. 21(1): p. 115-23. PMid:23214308

    PubMed/NCBI     
  29. Lonn, E., Do antioxidant vitamins protect against atherosclerosis? The proof is still lacking*. J Am Coll Cardiol, 2001. 38(7): p. 1795-8. 01626-6

    View Article           
  30. McQuillan, B.M., et al., Antioxidant vitamins and the risk of carotid atherosclerosis. The Perth Carotid Ultrasound Disease Assessment study (CUDAS). J Am Coll Cardiol, 2001. 38(7): p. 1788-94. 01676-X

    View Article           
  31. Malone, L., et al., Effect of estrogen and ovariectomy on response of the female rabbit urinary bladder to two forms of in vitro oxidative stress. Int Urogynecol J, 2014. 25(6): p. 791-8. PMid:24346814

    View Article      PubMed/NCBI     
  32. Lin, A.D., et al., Effect of bilateral in vivo ischemia/reperfusion on the activities of superoxide dismutase and catalase: response to a standardized grape suspension. Mol Cell Biochem, 2007. 296(1-2): p. 11-6. PMid:17203243

    View Article      PubMed/NCBI     
  33. Parekh, M.H., et al., Protective effect of vitamin E on the response of the rabbit bladder to partial outlet obstruction. J Urol, 2001. 166(1): p. 341-6. 66156-3

    View Article           
  34. Levin, R.M., et al., Effects of Ganoderma Lucidum shell-broken spore on oxidative stress of the rabbit urinary bladder using an in vivo model of ischemia/reperfusion. Mol Cell Biochem, 2017. PMid:28484937

    View Article      PubMed/NCBI     
  35. Levin, R.M., et al., Effect of oral Tadenan treatment on rabbit bladder structure and function after partial outlet obstruction. J Urol, 2002. 167(5): p. 2253-9. 65138-5

    View Article           
  36. Levin, R.M., et al., Effect of oral Kohki tea on bladder dysfunction induced by severe partial outlet obstruction. J Urol, 2002. 167(5): p. 2260-6. 65139-7

    View Article           
  37. Mohsin, M., P. Negi, and Z. Ahmed, Determination of the antioxidant activity and polyphenol contents of wild Lingzhi or Reishi medicinal mushroom, Ganoderma lucidum (W.Curt. Fr.) P. Karst. (higher Basidiomycetes) from central Himalayan hills of India. Int J Med Mushrooms, 2011. 13(6): p. 535-44. PMid:22181841

    View Article      PubMed/NCBI     
  38. Zhao, W., et al., Antioxidant activities of Ganoderma lucidum polysaccharides and their role on DNA damage in mice induced by cobalt-60 gamma-irradiation. Food Chem Toxicol, 2012. 50(2): p. 303-9. PMid:22079311

    View Article      PubMed/NCBI     
  39. Wong, K.L., et al., Antioxidant activity of Ganoderma lucidum in acute ethanol-induced heart toxicity. Phytother Res, 2004. 18(12): p. 1024-6. PMid:15742340

    View Article      PubMed/NCBI     
  40. Sun, J., H. He, and B.J. Xie, Novel antioxidant peptides from fermented mushroom Ganoderma lucidum. J Agric Food Chem, 2004. 52(21): p. 6646-52. PMid:15479035

    View Article      PubMed/NCBI     
  41. You, Y.H. and Z.B. Lin, [Antioxidant effect of Ganoderma polysaccharide peptide]. Yao Xue Xue Bao, 2003. 38(2): p. 85-8. PMid:12778739

    PubMed/NCBI     
  42. Bishop, K.S., et al., From 2000years of Ganoderma lucidum to recent developments in nutraceuticals. Phytochemistry, 2015. 114: p. 56-65. PMid:25794896

    View Article      PubMed/NCBI     
  43. Bean, H., et al., Antioxidant levels of common fruits, vegetables, and juices versus protective activity against in vitro ischemia/reperfusion. Int Urol Nephrol, 2010. 42(2): p. 409-15. PMid:19768567

    View Article      PubMed/NCBI     
  44. Haugaard, N., et al., Effect of partial obstruction of the rabbit urinary bladder on malate dehydrogenase and citrate synthase activity. J Urol, 1992. 147(5): p. 1391-3. 37580-8

    View Article           
  45. Spettel, S., et al., Citrate synthase, sarcoplasmic reticular calcium ATPase, and choline acetyltransferase activities of specific pelvic floor muscles of the rabbit. Mol Cell Biochem, 2012. 370(1-2): p. 1-5. PMid:22911511

    View Article      PubMed/NCBI     
  46. Levin, R.M., et al., Subcellular distribution of SERCA and calcium-activated ATPase in rabbit and human urinary bladder smooth muscle. Pharmacology, 1997. 55(3): p. 136-43. PMid:9346402

    View Article      PubMed/NCBI     
  47. Rogers, T.B., et al., Use of thapsigargin to study Ca2+ homeostasis in cardiac cells. Biosci Rep, 1995. 15(5): p. 341-9. PMid:8825036

    View Article      PubMed/NCBI     
  48. Barry, M., Meigs, JB, The natural history of benign prostatic hyperplasia, in Prostatic Diseases, H. Lepor, Editor. 2000, WB Saunders and Co.: Philadelphia. p. 106-115.

  49. Bushman, W., Etiology, epidemiology, and natural history of benign prostatic hyperplasia. Urol Clin North Am, 2009. 36(4): p. 403-15, v. PMid:19942041

    View Article      PubMed/NCBI     
  50. Guven, A., et al., Effect of age on the response to short-term partial bladder outlet obstruction in the rabbit. BJU Int, 2007. 100(4): p. 930-4. PMid:17822471

    View Article      PubMed/NCBI     
  51. Guven, A., et al., Effect of aging on the response of biochemical markers in the rabbit subjected to short-term partial bladder obstruction. Mol Cell Biochem, 2007. 306(1-2): p. 213-9. PMid:17673951

    View Article      PubMed/NCBI     
  52. Gosling, J.A., et al., Correlation between the structure and function of the rabbit urinary bladder following partial outlet obstruction. J Urol, 2000. 163(4): p. 1349-56. 67776-2

    View Article           
  53. Levin, R.M., et al., Obstructive response of human bladder to BPH vs. rabbit bladder response to partial outlet obstruction: a direct comparison. Neurourol Urodyn, 2000. 19(5): p. 609-29. 19:5<609::AID-NAU7>3.0.CO;2-H

    View Article           
  54. Levin, R.M., et al., Biochemical evaluation of obstructive bladder dysfunction in men secondary to BPH: a preliminary report. Urology, 1999. 53(2): p. 446-50. 00497-X

    View Article           
  55. Mannikarottu, A., et al., Effect of partial bladder outlet obstruction on nitrotyrosine levels and their correlation with contractile function. Neurourol Urodyn, 2006. 25(4): p. 397-401. PMid:16673378

    View Article      PubMed/NCBI     
  56. Juan, Y.S., et al., Coenzyme Q10 protect against ischemia/reperfusion induced biochemical and functional changes in rabbit urinary bladder. Mol Cell Biochem, 2008. 311(1-2): p. 73-80. PMid:18165912

    View Article      PubMed/NCBI     
  57. Malone, L., et al., The effect of in vitro oxidative stress on the female rabbit bladder contractile response and antioxidant levels. ISRN Urol, 2013. 2013: p. 639685. PMid:23819065

    PubMed/NCBI     
  58. Radu, F., et al., The effect of in vitro ischemia/reperfusion on contraction, free fatty acid content, phospholipid content, and malondialdehyde levels of the rabbit urinary bladder. Mol Cell Biochem, 2011. 346(1-2): p. 179-86. PMid:20882398

    View Article      PubMed/NCBI     
  59. Erdem, E., et al., Effect of maturation and aging on response of rabbit bladder to bilateral in vivo ischemia/reperfusion. Urology, 2006. 67(1): p. 220-4. PMid:16413379

    View Article      PubMed/NCBI     
  60. Bratslavsky, G., et al., Effects of in vivo ischemia on contractile responses of rabbit bladder to field stimulation, carbachol, ATP and KCl. Pharmacology, 1999. 59(4): p. 221-6. PMid:10474082

    View Article      PubMed/NCBI     
  61. Gill, H.S., et al., The effects of short-term in-vivo ischemia on the contractile function of the rabbit urinary bladder. J Urol, 1988. 139(6): p. 1350-4. 42917-X

    View Article           
  62. Yamada, A., et al., Persistent overexpression of SERCA2a affects bladder functions under physiological conditions, but not in bladder outlet obstruction-induced sub-acute pathological conditions. J Physiol Sci, 2014. 64(1): p. 21-30. PMid:24037709

    View Article      PubMed/NCBI     
  63. Callaghan, C.M., et al., The effect of partial outlet obstruction on calpain and phospholipase-2 activities: analyzed by severity and duration. Mol Cell Biochem, 2013. 381(1-2): p. 217-20. PMid:23737136

    View Article      PubMed/NCBI     
  64. Wang, Y., et al., Effects of mechanical stretch on interstitial cells of Cajal in guinea pig bladder. J Surg Res, 2010. 164(1): p. e213-9. PMid:20828727

    View Article      PubMed/NCBI     
  65. Zhao, Y., et al., Correlation of ischemia/reperfusion or partial outlet obstruction-induced spectrin proteolysis by calpain with contractile dysfunction in rabbit bladder. Urology, 1997. 49(2): p. 293-300. 00452-9

    View Article           
  66. Levin, R.M., et al., Bladder function in experimental outlet obstruction: pharmacologic responses to alterations in innervation, energetics, calcium mobilization, and genetics. Adv Exp Med Biol, 1995. 385: p. 7-19; discussion 75-9. PMid:8571847

    View Article      PubMed/NCBI     
  67. Stevenson, K., et al., Functional changes in bladder tissue from type III collagen-deficient mice. Mol Cell Biochem, 2006. 283(1-2): p. 107-14. PMid:16444592

    View Article      PubMed/NCBI     
  68. Eika, B., R.M. Levin, and P.A. Longhurst, Collagen and bladder function in streptozotocin-diabetic rats: effects of insulin and aminoguanidine. J Urol, 1992. 148(1): p. 167-72. 36546-1

    View Article           
  69. Monson, F.C., et al., Hyperplasia in the rabbit bladder urothelium following partial outlet obstruction. Autoradiographic evidence. Mol Cell Biochem, 1995. 152(2): p. 167-73. PMid:8751163

    View Article      PubMed/NCBI     
  70. Santarosa, R., et al., Hyperplasia and apoptosis. Opposing cellular processes that regulate the response of the rabbit bladder to transient outlet obstruction. Lab Invest, 1994. 70(4): p. 503-10. PMid:8176889

    PubMed/NCBI     
  71. Salomone, F., J. Godos, and S. Zelber-Sagi, Natural antioxidants for non-alcoholic fatty liver disease: molecular targets and clinical perspectives. Liver Int, 2016. 36(1): p. 5-20. PMid:26436447

    View Article      PubMed/NCBI     
  72. Kuno, A., M. Tanno, and Y. Horio, The effects of resveratrol and SIRT1 activation on dystrophic cardiomyopathy. Ann N Y Acad Sci, 2015. 1348(1): p. 46-54. PMid:26109180

    View Article      PubMed/NCBI     
  73. Conte, E., et al., Anti-inflammatory and antifibrotic effects of resveratrol in the lung. Histol Histopathol, 2015. 30(5): p. 523-9. PMid:25515609

    PubMed/NCBI     
  74. Juan, Y.S., et al., Coenzyme Q10 diminishes ischemia-reperfusion induced apoptosis and nerve injury in rabbit urinary bladder. Neurourol Urodyn, 2009. 28(4): p. 339-42. PMid:18837431

    View Article      PubMed/NCBI     
  75. Lasukova, T.V., et al., Cardioprotective Activity of Ganoderma lucidum Extract during Total Ischemia and Reperfusion of Isolated Heart. Bull Exp Biol Med, 2015. 158(6): p. 739-41. PMid:25896590

    View Article      PubMed/NCBI     
  76. Kan, Y., et al., Antioxidant activity of polysaccharide extracted from Ganoderma lucidum using response surface methodology. Int J Biol Macromol, 2015. 72: p. 151-7. PMid:25149043

    View Article      PubMed/NCBI     
  77. Liu, S.P., et al., Effects of hypoxia, calcium, carbachol, atropine and tetrodotoxin on the filling of the in-vitro rabbit whole bladder. J Urol, 1998. 160(3 Pt 1): p. 913-9. 62832-5

    View Article           
  78. Levin, R.M., et al., Etiology of bladder dysfunction secondary to partial outlet obstruction. Calcium disregulation in bladder power generation and the ability to perform work. Scand J Urol Nephrol Suppl, 1997. 184: p. 43-50. PMid:9165622

    PubMed/NCBI     
  79. Zhao, Y., et al., Effect of anoxia on in vitro bladder function. Pharmacology, 1991. 43(6): p. 337-44. PMid:1686111

    View Article      PubMed/NCBI     
  80. Tseng, C.Y., et al., Potent In Vitro Protection Against PM[Formula: see text]-Caused ROS Generation and Vascular Permeability by Long-Term Pretreatment with Ganoderma tsugae. Am J Chin Med, 2016. 44(2): p. 355-76. PMid:27080945

    View Article      PubMed/NCBI     
  81. Chang, C.J., et al., Ganoderma lucidum reduces obesity in mice by modulating the composition of the gut microbiota. Nat Commun, 2015. 6: p. 7489. PMid:26102296

    View Article      PubMed/NCBI     
  82. Shen, B., et al., An in vitro study of neuroprotective properties of traditional Chinese herbal medicines thought to promote healthy ageing and longevity. BMC Complement Altern Med, 2013. 13: p. 373. PMid:24373151

    View Article      PubMed/NCBI     
  83. Lai, C.S., et al., Antagonizing beta-amyloid peptide neurotoxicity of the anti-aging fungus Ganoderma lucidum. Brain Res, 2008. 1190: p. 215-24. PMid:18083148

    View Article      PubMed/NCBI     
  84. Cilerdzic, J., et al., Oxidative stress and species of genus Ganoderma (higher Basidiomycetes). Int J Med Mushrooms, 2013. 15(1): p. 21-8. PMid:23510281

    View Article      PubMed/NCBI     
  85. Weng, Y., et al., Ganodermasides C and D, two new anti-aging ergosterols from spores of the medicinal mushroom Ganoderma lucidum. Biosci Biotechnol Biochem, 2011. 75(4): p. 800-3. PMid:21512225

    View Article      PubMed/NCBI     
  86. Weng, Y., et al., Ganodermasides A and B, two novel anti-aging ergosterols from spores of a medicinal mushroom Ganoderma lucidum on yeast via UTH1 gene. Bioorg Med Chem, 2010. 18(3): p. 999-1002. PMid:20093034

    View Article      PubMed/NCBI     
  87. Sudheesh, N.P., et al., Therapeutic potential of Ganoderma lucidum (Fr.) P. Karst. against the declined antioxidant status in the mitochondria of post-mitotic tissues of aged mice. Clin Nutr, 2010. 29(3): p. 406-12. PMid:20044182

    View Article      PubMed/NCBI     
  88. Ajith, T.A., et al., Effect of Ganoderma lucidum on the activities of mitochondrial dehydrogenases and complex I and II of electron transport chain in the brain of aged rats. Exp Gerontol, 2009. 44(3): p. 219-23. PMid:19041385

    View Article      PubMed/NCBI     

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