doiSerbia

Inhibitory effect of propafenone derivatives on pseudomonas aeruginosa biofilm and pyocyanin production

Petrović Snježana (University of Belgrade, Institute for Medical Research, Centre of Research Excellence in Nutrition and Metabolism, Belgrade, Serbia)
Bašić Jasmina (Medical College of Applied Sciences in Belgrade, Zemun, Serbia)
Mandinić Zoran (University of Belgrade, School of Dental Medicine, Clinic for Pediatric and Preventive Dentistry, Belgrade, Serbia)
Božić Dragana D. (University of Belgrade, Faculty of Pharmacy, Department of Microbiology and Immunology, Belgrade, Serbia)
Milenković Marina (University of Belgrade, Faculty of Pharmacy, Department of Microbiology and Immunology, Belgrade, Serbia)
Vujić Zorica (University of Belgrade, Faculty of Pharmacy, Department of Pharmaceutical Chemistry, Belgrade, Serbia)

Introduction/Objective. Biofilm and pyocyanin production are essential components of Pseudomonas aeruginosa virulence and antibiotic resistance. Our objective was to examine inhibitory effect of synthetized propafenone derivatives 3-(2-Fluorophenyl)- 1-(2- (2-hydroxy-3-propylamino-propoxy)-phenyl)-propan-1-one hydrochloride (5OF) and3-(2- Trifluoromethyl-phenyl)-1-(2-(2-hydroxy-3-propylamino-propoxy)-phenyl)-propan-1-one hydrochloride (5CF3) on biofilm and pyocyanin in Pseudomonas aeruginosa clinical strains. Methods. Effects were tested on nine clinical isolates and one control laboratory strain of P. aeruginosa. In vitro analysis of biofilm growing was performed by incubating bacteria (0.5 McFarland) with 5OF and 5CF3 (500–31.2 μg/ml) and measuring optical density (OD) at 570 nm. Bacteria in medium without compounds were positive control. Blank medium (an uninoculated medium without test compounds) was used as negative control. Pyocyanin production was estimated by OD at 520 nm, after bacteria incubated with 5CF3 and 5OF (250 and 500 μg/ml), treated with chloroform, and chloroform layer mixed with HCl. Results. A total of 500 μg/ml of 5OF and 5CF3 completely inhibited biofilm formation in 10/10 and 4/10 strains, respectively. A total of 250 μg/ml of 5OF and 5CF3 strongly inhibited biofilm formation in 7/10 strains, while inhibition with 125 μg/ml of 5OF and 5CF3 was moderate. Lower concentrations had almost no effect on biofilm production. Pyocyanin production was reduced to less than 40% of the control value in 6/9, and less than 50% of the control in 7/9 strains with 500 μg/ml of 5OF and 5CF3, respectively. At 250 μg/ml 5OF and 5CF3, most strains had pyocyanin production above 50% of the control value. Conclusion. Synthetized propafenone derivatives, 5OF and 5CF3, inhibited biofilms and pyocyanin production of Pseudomonas aeruginosa clinical strains. Presented results suggest that propafenone derivatives are potential lead-compounds for synthesis of novel antipseudomonal drugs.

Keywords: Propafenone derivatives, Pseudomonas aeruginosa, biofilm, pyocyanin

Projects of the Serbian Ministry of Education, Science and Technological Development, Grant no. OI 172041 and Grant no. III 41030