Srpski arhiv za celokupno lekarstvo 2016 Volume 144, Issue 5-6, Pages: 280-287
https://doi.org/10.2298/SARH1606280S
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Cytotoxicity investigation of a new hydroxyapatite scaffold with improved structural design
Sjerobabin Nikola (Clinical Hospital Center “Dr Dragiša Mišović - Dedinje”, Belgrade)
Čolović Božana (Institute for Nuclear Sciences “Vinča”, Belgrade)
Petrović Milan (Faculty of Dental Medicine, Belgrade)
Marković Dejan (Faculty of Dental Medicine, Belgrade)
Živković Slavoljub (Faculty of Dental Medicine, Belgrade)
Jokanović Vukoman (Institute for Nuclear Sciences “Vinča”, Belgrade)
Introduction. Biodegradable porous scaffolds are found to be very promising
bone substitutes, acting as a temporary physical support to guide new tissue
regeneration, until the entire scaffold is totally degraded and replaced by
the new tissue. Objective. The aim of this study was to investigate
cytotoxicity of a synthesized calcium hydroxyapatite based scaffold, named
ALBO-OS, with high porosity and optimal topology. Methods. The ALBO-OS
scaffold was synthesized by the method of polymer foam template. The analysis
of pore geometry and scaffold walls’ topography was made by scanning electron
microscope (SEM). The biological investigations assumed the examinations of
ALBO-OS cytotoxicity to mouse L929 fibroblasts, using
3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromidefor (MTT) and
lactate dehydrogenase (LDH) tests and inverse phase microscopy. Results. The
SEM analysis showed high porosity with fair pore distribution and interesting
morphology from the biological standpoint. The biological investigations
showed that the material is not cytotoxic to L929 cells. Comparison of
ALBO-OS with Bio-Oss, as the global gold standard as a bone substitute,
showed similar results in MTT test, while LDH test showed significantly
higher rate of cell multiplication with ALBO-OS. Conclusion. The scaffold
design from the aspect of pore size, distribution, and topology seems to be
very convenient for cell adhesion and occupation, which makes it a promising
material as a bone substitute. The results of biological assays proved that
ALBO-OS is not cytotoxic for L929 fibroblasts. In comparison with Bio-Oss,
similar or even better results were obtained.
Keywords: hydroxyapatite, cytotoxicity, MTT, LDH
Projekat Ministarstva nauke
Republike Srbije, br. 172026