doiSerbia

Relationship between serum tumor necrosis factor receptor-2 concentration and periodontal destruction in patients with type 2 diabetes: Cross-sectional study

Matić-Petrović Sanja (Faculty of Dental Medicine, Department of Oral Medicine and Periodontology, Belgrade)
Pucar Ana (Faculty of Dental Medicine, Department of Oral Medicine and Periodontology, Belgrade)
Jotić Aleksandra (School of Medicine, Belgrade + Clinical Center of Serbia, Clinic of Endocrinology, Diabetes and Metabolic Disorders, Belgrade)
Miličić Biljana (Clinical Center of Serbia, Clinic of Endocrinology, Diabetes and Metabolic Disorders, Belgrade + Faculty of Dental Medicine, Department of Medical Informatics and Statistics, Belgrade)
Arambašić-Jovanović Jelena (Institute for Biological Research, Department of Molecular Biology, Belgrade)
Vidaković Melita (Institute for Biological Research, Department of Molecular Biology, Belgrade)
Leković Vojislav (Faculty of Dental Medicine, Department of Oral Medicine and Periodontology, Belgrade)

Introduction. The role of tumor necrosis factor-α (TNFα) is well documented in pathogenesis of chronic periodontitis (CP) and type 2 diabetes (T2D). Considering short half-life of TNFα, tumor necrosis factor receptor-2 (TNFR2) is used as prosperous surrogate marker of TNFα activity. Objective. The aim was to detect TNFR2 serum concentration and correlate it with periodontal destruction in patients with diagnosed T2D and nondiabetics. Methods. The study included 85 patients divided into three groups: T2D + CP (group T2D, n = 34); nondiabetics + CP (Group PD, n = 27); and healthy controls (group HC, n = 24). T2D was diagnosed according to WHO criteria (2013) and periodontitis was diagnosed using International Workshop for a Classification of Periodontal Diseases and Conditions criteria (1999). TNFR2 level was measured by enzyme-linked immunosorbent assay (ELISA). Results. There was no difference in TNFR2 level among the groups (Kruskal-Wallis, p = 0.482). Significant correlation (Pearson’s correlation coefficient) was observed between clinical attachment loss (CAL) and TNFR2 concentration in PD group (rp = -0.460, p = 0.016). In T2D group, correlations were observed between TNFR2 concentration and CAL (rp = 0.363, p = 0.005) and periodontal inflamed surface area (PISA) (rp = 0.345, p = 0.046) and periodontal epithelial surface area (PESA) (rp = 0.578, p = 0.000). Conclusion. Higher concentration of TNFR2 was associated with higher CAL, PESA, and PISA scores in T2D group. Contrary to that, nondiabetics with higher values of CAL exhibited lower concentration of TNFR2, presenting potential protective effect on periodontal destruction. These results imply that diabetes may alter TNFR2 secretion originated from periodontium.

Keywords: chronic periodontitis, TNFR2, TNF-alpha, type 2 diabetes

Projekat Ministarstva nauke Republike Srbije, br. 41008