Srpski arhiv za celokupno lekarstvo 2016 Volume 144, Issue 1-2, Pages: 10-14
https://doi.org/10.2298/SARH1602010B
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Estimation of total bacteria by real-time PCR in patients with periodontal disease
Brajović Gavrilo 
(Faculty of Dental Medicine, Department of Physiology, Belgrade)
Popović Branka (Faculty of Dental Medicine, Department of Human Genetics, Belgrade)
Puletić Miljan (Faculty of Dental Medicine, Clinic of Periodontology and Oral Medicine, Belgrade)
Kostić Marija (Faculty of Hotel Management and Tourism, Kragujevac)
Milašin Jelena (Faculty of Dental Medicine, Department of Human Genetics, Belgrade)
Introduction. Periodontal diseases are associated with the presence of
elevated levels of bacteria within the gingival crevice. Objective. The aim
of this study was to evaluate a total amount of bacteria in subgingival
plaque samples in patients with a periodontal disease. Methods. A
quantitative evaluation of total bacteria amount using quantitative real-time
polymerase chain reaction (qRT-PCR) was performed on 20 samples of patients
with ulceronecrotic periodontitis and on 10 samples of healthy subjects. The
estimation of total bacterial amount was based on gene copy number for 16S
rRNA that was determined by comparing to Ct values / gene copy number of the
standard curve. Results. A statistically significant difference between
average gene copy number of total bacteria in periodontal patients (2.55.107)
and healthy control (2.37.106) was found (p=0.01). Also, a trend of higher
numbers of the gene copy in deeper periodontal lesions (>7 mm) was confirmed
by a positive value of coefficient of correlation (r=0.073). Conclusion. The
quantitative estimation of total bacteria based on gene copy number could be
an important additional tool in diagnosing periodontitis.
Keywords: periodontitis, qPCR, total bacteria
Projekat
Ministarstva nauke Republike Srbije, br. 175075