Srpski arhiv za celokupno lekarstvo 2014 Volume 142, Issue 7-8, Pages: 457-463
https://doi.org/10.2298/SARH1408457C
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Phenotypic detection of beta-lactamases production in enterobacteriaceae
Ćirković Ivana (School of Medicine, Institute of Microbiology and Immunology, Belgrade)
Pavlović Ljiljana (Institute of Public Health of Serbia “Dr Milan Jovanović Batut”, Belgrade)
Konstantinović Neda (School of Medicine, Institute of Microbiology and Immunology, Belgrade)
Kostić Katarina (School of Medicine, Institute of Microbiology and Immunology, Belgrade)
Jovanović Snežana (Clinical Center of Serbia, Department of Microbiology, Belgrade)
Đukić Slobodanka (School of Medicine, Institute of Microbiology and Immunology, Belgrade)
Introduction. Beta-lactam antibiotics are the most commonly used
antibacterial drugs. However, many bacteria have developed resistance to
these antibiotics, and the most common form of resistance is the production
of beta-lactamase enzymes. Many members of the Enterobacteriaceae family
produce different types of these enzymes. Objective. The aim of this study
was to perform phenotypic detection of production and identification of
beta-lactamase type in Enterobacteriaceae isolated from different clinical
specimens from patients hospitalized in the Clinical Center of Serbia.
Methods. The strains of Enterobacteriaceae were collected between November
2011 and January 2012 in the laboratory of the Clinical Center of Serbia.
The isolates were identified according to the standard microbiology
procedures and confirmed by the Vitek2 automated system. Antimicrobial
susceptibility testing was performed by the disk diffusion method, and the
phenotypic detection of production and identification of betalactamases was
performed according to previously described methodologies. Results. In this
study, a total of 172 Enterobacteriaceae strains were isolated. Further
testing was performed on 54/145 (37.2%) strains showing decreased
susceptibility to beta-lactam antibiotics: 13/85 (15.3%) Escherichia coli,
31/46 (67.4%) Klebsiella pneumoniae and 10/14 (71.4%) Proteus mirabilis.
Among them, 40/145 (27.6%) strains produced extended spectrum betalactamases
(ESBLs), 9/145 (6.2%) - AmpC, 1/145 (0.7%) - K1 beta-lactamase and 4/145
(2.8%) - carbapenemases. Carbapenemases were predominantly detected in K.
pneumoniae (75%). Conclusion. Enterobacteriaceae produce different types of
betalactamases, and the most common type in our study was ESBLs. Production
of carbapenemases detected in Enterobacteriaceae is also an associated
problem.
Keywords: beta-lactamases, enterobacteria, resistance
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