Srpski arhiv za celokupno lekarstvo 2014 Volume 142, Issue 5-6, Pages: 301-305
https://doi.org/10.2298/SARH1406301Z
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Changes in subgingival microflora after placement and removal of fixed orthodontic appliances
Živković-Sandić Marija (School of Dental Medicine, Clinic for Orthodontics, Belgrade)
Popović Branka (School of Dental Medicine, Institute of Human Genetics, Belgrade)
Čarkić Jelena (School of Dental Medicine, Institute of Human Genetics, Belgrade)
Nikolić Nađa (School of Dental Medicine, Institute of Human Genetics, Belgrade)
Glišić Branislav (School of Dental Medicine, Clinic for Orthodontics, Belgrade)
Introduction. The placement of fixed orthodontic appliances may lead to
increased plaque accumulation and changes in subgingival microflora.
Objective. The aim of this study was to examine the changes in frequency of
subgingival microflora that occur after placement and removal of fixed
orthodontic appliance using polymerase chain reaction (PCR). Methods. This
study included 33 orthodontic patients, who were divided into two groups.
Subgingival plaque samples were collected from the right upper incisor (U1)
and right upper first molar (U6). In group A, the samples were taken three
times: before placement appliance (T1), after one month (T2), and after 3
months (T3). In group B the samples were also taken three times: before
appliance removal (T1), after one month (T2), and after three months (T3).
PCR method was used to determine the presence of P. gingivalis, A.
actinomycetemcomitans, T. forsythia, and P. intermedia. Results. In group A
the frequency of P. gingivalis showed statistically significant decrease at
U1 (p=0.049) and U6 (p=0.008), from T1 to T2, and at U1 (p=0.048) from T1 to
T3. In group B only the frequency of T. forsythia showed a statistically
significant decrease, at U6 (T1 vs. T2, p=0.004; T1 vs. T3, p=0.0003).
Regarding other analyzed bacteria, changes in the presence were noticed but
no statistical significance was found. Conclusion. Placement of fixed
appliances may have an impact on subgingival microflora, but in the first
months after the placement and removal of the appliance changes were not
significant, probably due to good oral hygiene.
Keywords: orthodontic appliance, bacteria, PCR
Projekat Ministarstva nauke
Republike Srbije, br. 175075