doiSerbia

Detection of herpes simplex virus type 1 in gingival crevicular fluid of gingival sulcus/periodontal pocket using polymerase chain reaction

Matić-Petrović Sanja (School of Dental Medicine, Department of Periodontology and Oral Medicine, Belgrade)
Zelić Ksenija (School of Medicine, Department of Anatomy, Laboratory of Anthropology, Belgrade)
Milašin Jelena (School of Dental Medicine, Institute of Human Genetics, Belgrade)
Popović Branka (School of Dental Medicine, Institute of Human Genetics, Belgrade)
Pucar Ana (School of Dental Medicine, Department of Periodontology and Oral Medicine, Belgrade)
Zelić Obrad (School of Dental Medicine, Department of Periodontology and Oral Medicine, Belgrade)

Introduction. Pathogenesis and some characteristics of periodontitis cannot be fully explained by bacterial etiology alone. Herpes viruses may bridge the gap between clinical characteristics and molecular understanding of periodontal destruction. Objective. The aim of this study was to investigate the prevalence of herpes simplex virus type 1 (HSV-1) in gingival crevicular fluid (GCF) of healthy and damaged periodontium in Serbian population and to explore potential correlation between the presence of this virus and the level of periodontal destruction. Methods. Samples were collected from gingival sulcus/periodontal pockets by sterile paper points and the presence of viral DNA in gingival crevicular fluid was assessed by PCR. Results. There was no statistically significant difference in HSV-1 in presence between periodontitis patients (PG=38.9%) and healthy controls (HC=32.3%), (Chi-square test, with Yates’ correction p=0.7574). However, HSV-1 positive patients showed significantly higher values of parameters of periodontal destruction (PPD=7.11±2.52, CAL=5.46±2.34) than periodontitis patients without HSV-1 in gingival crevicular fluid (PPD=4.70±1.79, CAL=3.39±2.65) (p values respectively, p=0.002 and p=0.023, Independent Samples T-Test). HSV-1 occurred more often in deeper (PPD≥6 mm) (69.2%) than in shallow pockets (3 mm

Keywords: periodontitis, herpes simplex, gingival crevicular fluid, periodontal pocket

Projekat Ministarstva nauke Republike Srbije, br. 45005 i br. 41008.