Srpski arhiv za celokupno lekarstvo 2013 Volume 141, Issue 9-10, Pages: 634-639
https://doi.org/10.2298/SARH1310634M
Full text ( 466 KB)
Production of pili, hemolysin and siderophores in the urinary isolates of Escherichia coli
Marković Tatjana (Javna zdravstvena ustanova, Institut za javno zdravstvo, Banja Luka, Republika Srpska)
Šmitran Aleksandra (Univerzitet u Banjoj Luci, Medicinski fakultet, Banja Luka, Republika Srpska)
Petković Miroslav (Univerzitet u Banjoj Luci, Medicinski fakultet, Banja Luka, Republika Srpska)
Introduction. Escherichia coli (E. coli) are the most frequent cause of the
urinary tract infections. Uropathogenic E. coli (UPEC) produce virulence
factors which enable them to survive in the urinary tract and cause an
infection. Objective. The objective of this study was to determine phenotype
characterization of E. coli isolated from outpatients’ urine in the region of
Banja Luka over three-year period. In line with the objective, the following
research tasks were set up: determining the production of type 1 fimbriae,
P-pili, α-hemolysin and siderophores. Methods. A total of 417 urinary
isolates and 100 control intestinal isolates were screened for virulence
factors. Production of adhesions was confirmed by haemagglutination test.
Plate haemolysis test was done for the detection of α-hemolysin, and
siderophores production assay was carried out by using the method named
chrome azurol sulfonate agar diffusion assay. Results. In the group of
urinary isolates, almost 60% of isolates produced two or three virulence
factors; only 3.8% produced none of the virulence factors. In the group of
intestinal isolates, even 43% of isolates produced none of the virulence
factors while 48% of isolates produced a single virulence factor and 9%
produced two virulence factors. Conclusion. Urinary isolates E. coli express
significantly more P-pili, α-hemolysin and siderophore than intestinal
isolates (p<0.001). There was no significant difference in production of type
1 fimbriae among the urinary and intestinal isolates.
Keywords: uropathogenic Escherichia coli, bacterial adhesions, iron chelating agents