Srpski arhiv za celokupno lekarstvo 2013 Volume 141, Issue 9-10, Pages: 629-633
https://doi.org/10.2298/SARH1310629G
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Genetic polymorphisms of paraoxonase 1 and susceptibility to atherogenesis
Grubiša Ivana (Kliničko-bolnički centar „Zvezdara“, Odeljenje za humanu genetiku i prenatalnu dijagnostiku, Beograd)
Otašević Petar (Medicinski fakultet, Beograd + Institut za kardiovaskularne bolesti „Dedinje“, Beograd)
Dimković Nada (Medicinski fakultet, Beograd + Kliničko-bolnički centar „Zvezdara“, Kliničko odeljenje za bubrežne bolesti, Beograd)
Nedeljković Ivana (Stomatološki fakultet, Institut za humanu genetiku, Beograd)
Toljić Boško (Stomatološki fakultet, Institut za humanu genetiku, Beograd)
Vučinić Nada (Kliničko-bolnički centar „Zvezdara“, Odeljenje za humanu genetiku i prenatalnu dijagnostiku, Beograd)
Introduction. Paraoxonase 1 (PON1) is a multifunctional enzyme associated
with high-density lipoprotein particles (HDL). It is a cellular antioxidant
that hydrolyses oxidized macromolecules, especially low-density lipoproteins
(ox-LDL). Because increased oxidative stress is believed to play a crucial
role in the initiation and propagation of atherosclerosis, coding (Q192R and
L55M) and promoter (C(-107)T) region polymorphisms of pon1 gene, that are
responsible for catalytic efficiency, activity and the level of the enzyme,
have been of great interest as a potential markers of susceptibility for
atherogenesis. Objective. The aim of the study was to assess possible
association between these pon1 gene variants and clinical manifestations of
the atherosclerosis and oxidative stress. Methods. A total of 60
angiographically documented patients with manifested atherosclerotic disease
and 100 control individuals were analyzed. Genomic DNA was isolated from the
peripheral blood cells and genotyping was performed using polymerase chain
reaction followed by the restriction fragment length polymorphism (PCR-RFLP)
analysis. Results No significant difference in allele and genotype
frequencies of all three examined polymorphisms was found between the
atherosclerotic patients and healthy controls. The obtained results could not
support an association of pon1 gene variants with the oxidative stress and
atherogenesis. Conclusion. These polymorphisms cannot be considered risk
factors of atherosclerosis in Serbian population. A larger study is required
in order to establish possible contribution of pon1 variants to
atherosclerosis-related cardiovascular diseases.
Keywords: paraoxonase 1, gene polymorphisms, oxidative stress, atherogenesis
Projekat Ministarstva nauke
Republike Srbije, br. 175075