Srpski arhiv za celokupno lekarstvo 2013 Volume 141, Issue 9-10, Pages: 602-607
https://doi.org/10.2298/SARH1310602K
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Peutz-Jeghers syndrome: Quantitative study on enterochromaffin cells in hamartomatous intestine polyps
Krstić Miljan (Medicinski fakultet, Institut za patologiju, Niš)
Katić Vuka (Medicinski fakultet, Institut za patologiju, Niš)
Stojnev Slavica (Medicinski fakultet, Institut za patologiju, Niš)
Mihailović Dragan (Medicinski fakultet, Institut za patologiju, Niš)
Mojsilović Marijola (Medicinski fakultet, Institut za histologiju, Niš)
Radovanović Zoran (Medicinski fakultet, Institut za radiologiju, Niš)
Živković Vladimir (Medicinski fakultet, Institut za anatomiju, Niš)
Introduction. Peutz-Jeghers (PJ) syndrome is a rare familial disorder with
the autosomal transmission characterized by multiple intestinal polyps,
mucocutaneous pigmentation and increased incidence of various malignancies.
Some clinical manifestations of PJ syndrome may be associated with the
serotonin secretion from the enterochromaffin cells (EC). Objective. Since no
data have been reported so far regarding EC cells in PJ polyps, the aim of
our study was to quantitatively investigate EC population in hamartomatous
intestinal polyps in patients with the PJ syndrome. Methods. The samples of
surgically removed PJ polyps from family members with the PJ syndrome were
collected during 34-year follow-up period. Formalin-fixed paraffin-embedded
specimens of twenty-one PJ polyps were stained with HE, AB-PAS, Van Gieson,
Fontana-Masson, FIF and Grimelius. For immuno- histochemical analysis, the
following antibodies were used: chromogranin A, serotonin, Ki-67, desmin,
vimentin and cytokeratin in order to eliminate differential diagnostic
possibilities and to confirm diagnosis of PJ polyps. Results. Strong EC cell
hyperplasia was observed within the tissue of the investigated polyps.
Statistical analysis demonstrated significantly higher content of EC cells in
PJ polyps than in the normal ileal mucosa. Conclusion. Marked hyperplasia of
EC cells within the PJ polyps may be the most important contributor to
functional disorders in patients with the PJ syndrome.
Keywords: Peutz-Jeghers syndrome, hamartomatous polyp, enterochromaffin cells, chromogranin A
Projekat Ministarstva
nauke Republike Srbije, br. 175092