doiSerbia

Collagen type I alpha 1 gene polymorphism in premature ovarian failure

Vujović Svetlana (Medicinski fakultet, Beograd + Klinički centar Srbije, Klinika za endokrinologiju, dijabetes i bolesti metabolizma, Beograd)
Kanazir Selma (Institut za biološka istraživanja, Odeljenje za neurobiologiju, Beograd)
Ivović Miomira (Medicinski fakultet, Beograd + Klinički centar Srbije, Klinika za endokrinologiju, dijabetes i bolesti metabolizma, Beograd)
Tančić-Gajić Milina (Medicinski fakultet, Beograd + Klinički centar Srbije, Klinika za endokrinologiju, dijabetes i bolesti metabolizma, Beograd)
Perović Milka (Institut za biološka istraživanja, Odeljenje za neurobiologiju, Beograd)
Baltić Svetlana (Institut za biološka istraživanja, Odeljenje za neurobiologiju, Beograd)
Marina Ljiljana (Medicinski fakultet, Beograd + Klinički centar Srbije, Klinika za endokrinologiju, dijabetes i bolesti metabolizma, Beograd)
Barać Marija (Medicinski fakultet, Beograd + Klinički centar Srbije, Klinika za endokrinologiju, dijabetes i bolesti metabolizma, Beograd)
Ivanišević Maja (Medicinski fakultet, Beograd + Klinički centar Srbije, Klinika za ginekologiju i akušerstvo, Beograd)
Micić Jelena (Medicinski fakultet, Beograd + Klinički centar Srbije, Klinika za ginekologiju i akušerstvo, Beograd)
Micić Dragan (Medicinski fakultet, Beograd + Klinički centar Srbije, Klinika za endokrinologiju, dijabetes i bolesti metabolizma, Beograd)

Introduction. Premature ovarian failure (POF) is characterized by amenorrhea, hypergonadotropism and hypoestrogenism in women bellow 40 years. Osteoporosis is one of the late complications of POF. Objective. To correlate collagen type I alpha1 (COLIA1) gene polymorphism with bone mineral density (BMD) in women with POF. Methods. We determined the COLIA1 genotypes SS, Ss, ss in 66 women with POF. Single nucleotide polymorphism (G to T substitution) within the Sp 1-binding site in the first intron of the COLIA1 gene was assessed by polymerase chain reaction (PCR) followed by single-stranded conformation polymorphism (SSCP) analysis. Bone mineral density (BMD) was measured at the lumbar spine region by dual X-ray absorptiometry. Statistics: Kruskal-Wallis ANOVA, Chisquare test, Spearman correlation test. Results. The relative distribution of COLIA1 genotype alleles was SS - 54.4%, Ss - 41.0% and ss - 4.5%. No significant differences were found between genotype groups in body mass index, age, duration of amenorrhea or BMD. A significant positive correlation was observed between BMI and parity. Conclusion. The COLIA1 gene is just one of many genes influencing bone characteristics. It may act as a marker for differences in bone quantity and quality, bone fragility and accelerated bone loss in older women. However, in young women with POF, COLIA1 cannot identify those at higher risk for osteoporosis.

Keywords: COLIA1, genetic polymorphism, premature ovarian failure, osteoporosis

Projekat Ministarstva nauke Republike Srbije, br. ON 173056