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Srpski arhiv za celokupno lekarstvo 2013 Volume 141, Issue 3-4, Pages: 178-186
https://doi.org/10.2298/SARH1304178T
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Mesenchymal stem cells isolated from peripheral blood and umbilical cord Wharton’s jelly

Trivanović Drenka ORCID iD icon (Institut za medicinska istraživanja, Laboratorija za eksperimentalnu hematologiju i matične ćelije, Beograd)
Kocić Jelena (Institut za medicinska istraživanja, Laboratorija za eksperimentalnu hematologiju i matične ćelije, Beograd)
Mojsilović Slavko ORCID iD icon (Institut za medicinska istraživanja, Laboratorija za eksperimentalnu hematologiju i matične ćelije, Beograd)
Krstić Aleksandra (Institut za medicinska istraživanja, Laboratorija za eksperimentalnu hematologiju i matične ćelije, Beograd)
Ilić Vesna ORCID iD icon (Institut za medicinska istraživanja, Laboratorija za imunologiju, Beograd)
Okić-Đorđević Ivana ORCID iD icon (Institut za medicinska istraživanja, Laboratorija za eksperimentalnu hematologiju i matične ćelije, Beograd)
Santibanez Juan Francisco (Institut za medicinska istraživanja, Laboratorija za eksperimentalnu hematologiju i matične ćelije, Beograd)
Jovčić Gordana (Institut za medicinska istraživanja, Laboratorija za eksperimentalnu hematologiju i matične ćelije, Beograd)
Terzić Milan ORCID iD icon (Klinika za ginekologiju i akušerstvo, Klinički centar Srbije, Beograd + Medicinski fakultet, Beograd)
Bugarski Diana (Institut za medicinska istraživanja, Laboratorija za eksperimentalnu hematologiju i matične ćelije, Beograd)

Introduction. Mesenchymal stem cells (MSCs) are a promising tool for regenerative medicine, but due to the heterogeneity of their populations, different sources and isolation techniques, the characteristics defining MSCs are inconsistent. Objective. The aim of this study was to compare the characteristics of MSCs derived from two different human tissues: peripheral blood (PB-MSCs) and umbilical cord Wharton’s Jelly (UC-MSCs). Methods. The PB-MSC and UC-MSC were isolated by adherence to plastic after gradient-density separation or an explant culture method, respectively, and compared regarding their morphology, clonogenic efficiency, proliferating rates, immunophenotype and differentiation potential. Results. MSCs derived from both sources exhibit similar morphology, proliferation capacity and multilineage (osteogenic, chondrogenic, adipogenic and myogenic) differentiation potential. Differences were observed in the clonogenic capacity and the immunophenotype, since UC-MSCs showed higher CFU-F (colony-forming units-fibroblastic) cloning efficiency, as well as higher embryonic markers (Nanog, Sox2, SSEA4) expression. When additional surface antigens were analyzed by flow cytometry (CD44, CD90, CD105, CD33, CD34, CD45, CD11b, CD235a) or immunofluorescent labeling (vimentin, STRO-1 and α-smooth muscle actin), most appeared to have similar epitope profiles irrespective of MSC source. Conclusion. The results obtained demonstrated that both MSCs represent good alternative sources of adult MSCs that could be used in cell therapy applications.

Keywords: mesenchymal stem cells, peripheral blood, umbilical cord, characterization

Projekat Ministarstva nauke Republike Srbije, br. 175062