Srpski arhiv za celokupno lekarstvo 2013 Volume 141, Issue 3-4, Pages: 169-172
https://doi.org/10.2298/SARH1304169R
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Tumor necrosis factor alpha gene polymorphism in Serbian patients with sarcoidosis
Rađenović-Petković Tatjana (Klinički centar, Klinika za plućne bolesti, Niš)
Pejčić Tatjana (Klinički centar, Klinika za plućne bolesti, Niš)
Videnović-Ivanov Jelica (Klinički centar Srbije, Klinika za plućne bolesti, Beograd)
Jevtović-Stoimenov Tatjana (Medicinski fakultet, Laboratorija za funkcionalnu genomiku i proteomiku, Niš)
Janković Irena (Medicinski fakultet, Centar za medicinsku statistiku, Niš)
Nastasijević-Borovac Desa (Klinički centar, Klinika za plućne bolesti, Niš)
Radojković Danijela (Klinički centar, Klinika za endokrinologiju, Niš)
Introduction. Sarcoidosis is a multisystemic disease of unknown etiology.
Genetic factors play a considerable role in the onset of the disease. Tumor
necrosis factor alpha (TNF-α) is a proinflammatory cytokine which plays an
important role in the pathogenesis of the disease and the formation of
granuloma by regulating cellular proliferation and apoptosis. Objective. The
aim of this study was to investigate the role of TNF-α-308 G/A polymorphism
in the development of sarcoidosis and to evaluate the association between the
aforementioned type of polymorphism and the clinical course of the disease.
Methods. Seventy patients with sarcoidosis and 50 healthy volunteers were
genotyped for the TNF-α-308G/A polymorphism. Polymorphism variants were
examined by PCR-RFLP (polymerase chain reaction-restriction fragment length
polymorphism) on the DNA isolated from blood leukocytes. Results. There were
no significant differences in TNF-α-308A allele frequency distribution
between sarcoidosis patients and the control group, but the TNF-α-308A allele
was observed significantly more frequently in the sarcoidosis patients with
Löfgren’s syndrome when compared with non-Löfgren’s patients. Conclusion. We
have found that the TNF-α-308A variant is associated with Löfgren’s syndrome
in Serbian patients with sarcoidosis.
Keywords: sarcoidosis, gene, polymorphism, TNF-α