Srpski arhiv za celokupno lekarstvo 2013 Volume 141, Issue 3-4, Pages: 155-162
https://doi.org/10.2298/SARH1304155M
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Analysis of antimicrobial effect of MTAD solution in infected canal system using PCR technique
Mitić Aleksandar (Medicinski fakultet, Klinika za stomatologiju, Odeljenje za bolesti zuba i endodonciju, Niš)
Mitić Nadica (Medicinski fakultet, Klinika za stomatologiju, Odeljenje za bolesti zuba i endodonciju, Niš)
Milašin Jelena (Stomatološki fakultet, Institut za humanu genetiku, Beograd)
Živković Slavoljub (Stomatološki fakultet, Klinika za bolesti zuba i endodonciju, Beograd)
Gašić Jovanka (Medicinski fakultet, Klinika za stomatologiju, Odeljenje za bolesti zuba i endodonciju, Niš)
Mitić Vladimir (Medicinski fakultet, Klinika za stomatologiju, Odeljenje za ortopediju vilica, Niš)
Popović Jelena (Medicinski fakultet, Klinika za stomatologiju, Odeljenje za bolesti zuba i endodonciju, Niš)
Introduction. Clinically acceptable antiseptic should possess
organolithic-mineralolithic properties and antimicrobial efficacy, and should
be non-toxic. Objective. The aim of the paper was to assess the presence of
genomes of the most common microorganisms (Porphyromonas gingivalis,
Agregatibacter actinomycetemcomitans, Tanerella forsythensis, Prevotella
intermedia, Treponema denticola and Enterococcus faecalis) in infected tooth
root canals before and after rinsing with solution of doxycycline, citric
acid and detergent Tween-80 (MTAD) in patients with clinically diagnosed
primary apex periodontitis. Methods. The content of primarily infected canals
before and after using the MTAD solution was used as a biological material in
which the presence of microorganisms DNA was proved. For the detection of
bacterial genome the multiplex PCR technique was applied. Results. The
percentage of positive samples before canal treatment was 100%. In infected
root canals E. faecalis was most dominant (37%). In a relatively high
percentage we detected P. intermedia (25%), A. actinomycetemcomitans (20%),
T. denticola (17%), T. forsythensis (15%) and P. gingivalis (10%). After
rinsing the canal system using MTAD solution, there was a statistically
significant decrease in E. faecalis (12%), P. intermedia (0%), T.
forsythensis (0%) and P. gingivalis (0%). The presence of other bacteria was
also diminished but not statistically significantly. Conclusion. With the
application of multiplex PCR technique which provided a simultaneous
amplification of various genomic sequences, using several pairs of primers,
the most dominant in infected root canals were E. faecalis. P. intermedia, A.
actinomycetemcomitans, T. denticola, T. forsythensis and P. gingivalis. After
mechanic treatment and irrigation of root canals with MTAD solution, P.
intermedia, P. gingivalis and T. forsythensis were not found. The presence of
E. faecalis, A. actinomycetemcomitans and T. denticola was diminished,
however, not statistically significantly.
Keywords: infection, root canal, endopathogenic bacteria, PCR