Srpski arhiv za celokupno lekarstvo 2010 Volume 138, Issue 9-10, Pages: 577-583
https://doi.org/10.2298/SARH1010577S
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Influence of metabolism modifiers of cyclic nucleotides on contractility of right ventricle of rat heart with intact and removed endocardial endothelium
Smiljić Sonja
Radović Dragica
Miletić Milanka
Nestorović Vojkan
Trajković Goran
Savić Slađana
Introduction. Endocardial endothelium, a natural biological barrier between
circulating blood in heart ventricle and cells, creates a complex yet finely
tuned balance of interactions with the immediate environment. Objective. We
investigated the roles of theophylline, nonspecific phosphodiesterase
inhibitor, and imidazole, an activator of phosphodiesterase on contractility
of the right ventricle of rat heart, with intact and removed endocardial
endothelium. Methods. Adult rats, of both sexes, type Wistar albino, were
used in this experiment. All experiments were conducted on the preparations
of the right ventricle using two experimental models. In the first
experimental model, an endocardial endothelium (EE) was preserved, and in the
second model, an endocardial endothelium (-EE) was removed using 1% solution
Triton X-100. Results. Theophylline (1x10-2 mol/l) expressed the positive
inotropic effect on the heart, regardless of the presence of the endocardial
endothelium. Inotropic response as multiple process can be induced by
inhibition of phosphodiesterase, accumulation of cyclic nucleotides and
activation of Ca2+ channels. Imidazole (2x10-3 mol/l) increased the
contractility of the right ventricle of the heart with EE. The modulator
effect of endocardial endothelium on contractility of imidazole proved to be
significant. As imidazole influenced the contractility of the right ventricle
only in the presence of the endocardial endothelium, it is assumed that its
effect is mediated via deliverance of endothelial mediators with positive
inotropic effect. Conclusion. An intact endocardial endothelium is necessary
for completion of contractile performance of the heart.
Keywords: endocardial endothelium, cAMP, phosphodiesterase, theophylline, imidazole
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