doiSerbia

Identification of periodontopathogen microorganisms by PCR technique

Milićević Radovan (Dečja klinika, Klinički centar, Niš)
Brajović Gavrilo (Institut za fiziologiju, Stomatološki fakultet, Beograd)
Nikolić-Jakoba Nataša (Klinika za parodontologiju i oralnu medicinu, Stomatološki fakultet, Beograd)
Popović Branka (Institut za humanu genetiku, Stomatološki fakultet, Beograd)
Pavlica Dušan (Institut za mikrobiologiju, Stomatološki fakultet, Beograd)
Leković Vojislav (Klinika za parodontologiju i oralnu medicinu, Stomatološki fakultet, Beograd)
Milašin Jelena (Institut za humanu genetiku, Stomatološki fakultet, Beograd)

INTRODUCTION Periodontitis is an inflammatory disease of the supporting tissues of teeth and is a major cause of tooth loss in adults. The onset and progression of periodontal disease is attributed to the presence of elevated levels of a consortium of pathogenic bacteria. Gram negative bacteria, mainly strict anaerobes, play the major role. OBJECTIVE The present study aimed to assess the presence of the main types of microorganisms involved in the aetiopathogenesis of periodontal disease: Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Eikenella corrodens, Treponema denticola, Tanerella forsythia and Prevotella intermedia in different samples collected from the oral cavity of 90 patients diagnosed with periodontitis. METHOD Bacterial DNA detection was performed in diverse biological materials, namely in dental plaque, gingival tissue and saliva, by means of multiplex PCR, a technique that allows simultaneous identification of two different bacterial genomes. RESULTS In the dental plaque of the periodontitis patients, Treponema denticola dominated. In the gingival tissue, Tannerella forsythia and Treponema denticola were the microbiota most frequently detected, whilst in saliva Treponema denticola and Eikenella corrodens were found with the highest percentage. CONCLUSION The identification of microorganisms by multiplex PCR is specific and sensitive. Rapid and precise assessment of different types of periodontopathogens is extremely important for early detection of the infection and consequently for the prevention and treatment of periodontal disease. In everyday clinical practice, for routine bacterial evaluation in patients with periodontal disease, the dental plaque is the most suitable biological material, because it is the richest in periodontal bacteria.

Keywords: dental plaque, saliva, periodontal tissue, periodontopathogens, PCR