Chemical Industry and Chemical Engineering Quarterly 2015 Volume 21, Issue 1-2, Pages: 201-210
https://doi.org/10.2298/CICEQ140228031H
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Review-evaluating the molecular assays for measuring the oxidative potential of particulate matter
Hedayat F. (ILAQH, Queensland University of Technology, Brisbane, Australia)
Stevanovic S. (ILAQH, Queensland University of Technology, Brisbane, QLD, Australia + ARC Centre of Excellence for Free Radical Chemistry and Biotechnology, QUT, Brisbane, Australia)
Miljevic B. (ILAQH, Queensland University of Technology, Brisbane, Australia)
Bottle S. (ARC Centre of Excellence for Free Radical Chemistry and Biotechnology, QUT, Brisbane, Australia)
Ristovski Z.D. (ILAQH, Queensland University of Technology, Brisbane, QLD, Australia + Biofuel Engine Research Facility, QUT, Brisbane, Australia)
Several cell-free assays are currently used to quantify and detect the
Reactive Oxygen Species (ROS). All of them have certain limitations, do not
provide direct comparison of results and, to date, none of these assays have
been acknowledged as the most suitable acellular assay and none has yet been
adopted for investigation of potential PM toxicity. These assays include DTT,
ascorbic acid, DCFHDA and PFN assays which have been used in measurements of
the particles generated from various combustion sources such as diesel
engine, wood smoke (or biomass burning) and cigarette smoke, as well as for
outdoor measurements. All the probes use different units for expressing redox
properties of PM. Also, their reactivity is being triggered by different
types of ROS. This limits the direct comparison of the results that are
reporting the toxicity of the same aerosol type measured with various probes.
This study is evaluating and comparing the various assays in order to develop
deeper understanding of their capabilities, selectivity as well as improve
understanding of the underlying chemical mechanisms.
Keywords: DTT, DCFH-DA, PFN, BPEA-nit, Ascorbic acid, oxidative potential