Volume 4, 2020

Biophysics

LABORATORY EXAMINATION TECHNIQUE FOR ANIMAL SKIN DERIVATIVES (HAIR, WOOL, FLUFF)

A.A. Oleshkevich, S.A. Komarova, A.A. Guselnikova, E.I. Yarygina

Pages: 95-100

DOI: 10.21175/RadProc.2020.20

The hair (one of skin derivatives) structure is polymorphic, therefore the study of its specific features has diagnostic value. Nowadays, the main methods for the wool species determining are either microscopy of hair in transmitted light, or light microscopy. The relevance of the work is caused by the need to study hair and its alkaline hydrolysates using the simplest, fastest and most modern biophysical methods in order to create a laboratory test system in the future. To determine the species of animal hair, the possibility of using of the following biophysical methods was studied: polarization-interference microscopy, reflected light microscopy, fluorescence microscopy, ultraviolet spectrophotometry, and redox-measurement. Hair of different color, shape, length and thickness from animals of various classes, families and species were selected as samples. The possibilities of each method, their advantages and disadvantages were studied in samples selected. It is shown that the effectiveness of luminescence microscopy and reflected light microscopy are limited, and techniques cannot be recognized as reliably informative. The method of polarization-interference microscopy is one the most promising and can serve as alternative to reflected light microscopy. The possibility of the new redox-measurement method has been revealed. The authors have developed a special device for redox measurements and tested it in laboratory practice. The probability of the presence of a different number of absorption bands in the absorption spectra (hair species from various animals) is proven by spectrophotometric method. This allows to use ultraviolet spectrophotometry for the hair species identification. According to the authors, the most informative in laboratory identification of animal hair samples will be provided using the ultraviolet spectrophotometry method combined with the redox-potential measurement in alkaline wool hydrolysates.
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A.A. Oleshkevich , S.A. Komarova, A.A. Guselnikova, E.I. Yarygina, "Laboratory examination technique for animal skin derivatives (hair, wool, fluff)," RAD Conf. Proc, vol. 4, 2020, pp. 95–100, http://doi.org/10.21175/RadProc.2020.20