Macromolecular Crystallography Protocols, Volume 2

1. Front Matter

   Pages i-xii

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2. Cryocrystallography of Macromolecules

   • Elspeth Garman, Robin L. Owen

   Pages 1-18

3. Determination of Reaction Intermediate Structures in Heme Proteins

   • Kelvin Chu

   Pages 19-29

4. Annealing Macromolecular Crystals

   • B. Leif Hanson, Gerard J. Bunick

   Pages 31-41

5. First Analysis of Macromolecular Crystals

   • David Jeruzalmi

   Pages 43-61

6. X-Ray Data Collection From Macromolecular Crystals

   • Elspeth Garman, Robert M. Sweet

   Pages 63-93

7. Characterizing a Crystal From an Initial Native Dataset

   • Michael R. Sawaya

   Pages 95-120

8. Molecular Replacement

   • Eric A. Toth

   Pages 121-147

9. Phase Determination Using Halide Ions

   • Miroslawa Dauter, Zbigniew Dauter

   Pages 149-158

10. The Same But Different

    • Mark A. Rould

    Pages 159-181

11. Substructure Determination in Multiwavelength Anomalous Diffraction, Single Anomalous Diffraction, and Single Isomorphous Replacement With Anomalous Scattering Data Using Shake-and-Bake

    • G. David Smith, Christopher T. Lemke, P. Lynne Howell

    Pages 183-195

12. Substructure Determination in Isomorphous Replacement and Anomalous Diffraction Experiments

    • Ralf W. Grosse-Kunstleve, Thomas R. Schneider

    Pages 197-214

13. Automated Structure Solution With autoSHARP

    • Clemens Vonrhein, Eric Blanc, Pietro Roversi, Gérard Bricogne

    Pages 215-230

14. Introduction to Macromolecular Refinement

    • Dale E. Tronrud

    Pages 231-253

15. Quality Control and Validation

    • Gerard J. Kleywegt

    Pages 255-272

16. Crystallographic Software

    • Stephen J. Everse, Sylvie Doublié

    Pages 273-278

17. Back Matter

    Pages 279-283

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In the decade since publication of the first edition of Crystallographic Methods and Protocols the field has seen several major developments that have both accelerated the pace of structure determination and made crystallography accessible to a broader range of investigators. Volume I, Preparation and Crystallization of Macromolecules is dedicated to the crystallization and ways to increase the odds of obtaining crystals in macromolecules, while Volume 2, Structure Determination, covers both computational methods for characterizing crystals and solving structures.

• Endoplasmatisches Reticulum
• Polyacrylamid-Gelelektrophorese
• Termination
• biochemistry
• proteins

• Department of Microbiology and Molecular Genetics, University of Vermont, Burlingon

  Sylvie Doublié

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