Arabidopsis Protocols, 2nd Edition

1. Front Matter

   Pages i-xv

   PDF

2. Growing Arabidopsis

   1. Front Matter

      Pages 1-1

      PDF

   2. Growth of Plants and Preservation of Seeds

      • Luz Rivero-Lepinckas, Deborah Crist, Randy Scholl

      Pages 3-12

   3. Prevention and Control of Pests and Diseases

      • Jenifer Bush, Georg Jander, Frederick M. Ausubel

      Pages 13-25

   4. Growth of Other Species Related to Arabidopsis thaliana

      • Krithika Yogeeswaran, Mikhail Elia Nasrallah

      Pages 27-38

   5. Grafting

      • Katherine Bainbridge, Tom Bennett, Colin Turnbull, Ottoline Leyser

      Pages 39-44

   6. Synchronization, Transformation, and Cryopreservation of Suspension-Cultured Cells

      • Margit Menges, James A. H. Murray

      Pages 45-61

3. Genetic Analyses

   1. Front Matter

      Pages 63-63

      PDF

   2. Genetic Analysis

      • Maarten Koornneef, Carlos Alonso-Blanco, Piet Stam

      Pages 65-77

   3. QTL Analysis

      • Carlos Alonso-Blanco, Maarten Koornneef, Johan W. van Ooijen

      Pages 79-99

   4. EMS Mutagenesis of Arabidopsis

      • YongSig Kim, Karen S. Schumaker, Jian-Kang Zhu

      Pages 101-103

   5. Low-Resolution Mapping of Untagged Mutations

      • M. Rosa Ponce, Pedro Robles, Francisca M. Lozano, M. Asunción Brotóns, José L. Micol

      Pages 105-113

   6. Gene Identification and Cloning by Molecular Marker Mapping

      • Georg Jander

      Pages 115-126

   7. High-Throughput TILLING for Arabidopsis

      • Bradley J. Till, Trenton Colbert, Christine Codomo, Linda Enns, Jessica Johnson, Steven H. Reynolds et al.

      Pages 127-135

   8. Genotyping and Mapping With High-Density Oligonucleotide Arrays

      • Justin Borevitz

      Pages 137-145

   9. Forward Genetic Screening of Insertional Mutants

      • Anja Schneider, Dario Leister

      Pages 147-161

   10. PCR-Based Screening for Insertional Mutants

       • Anna N. Stepanova, Jose M. Alonso

       Pages 163-172

   11. Cytogenetic Analyses of Arabidopsis

       • Martin Lysak, Paul Fransz, Ingo Schubert

       Pages 173-186

   12. Using Information From Public Arabidopsis Databases to Aid Research

       • Margarita Garcia-Hernández, Leonore Reiser

       Pages 187-211

4. Transient and Stable Transformation

   1. Front Matter

      Pages 213-213

      PDF

For several decades, Arabidopsis thaliana has been the organism of choice in the laboratories of many plant geneticists, physiologists, developmental biologists, and biochemists around the world. During this time, a huge amount of knowledge has been acquired on the biology of this plant species, which has resulted in the development of molecular tools that account for much more efficient research. The significance that Arabidopsis would attain in biological research may have been difficult to foresee in the 1980s, when its use in the laboratory started. In the meantime, it has become the model plant organism, much the same way as Drosophila, Caenorhabditis, or mouse have for animal systems. Today, it is difficult to envision research at the cutting edge of plant biology without the use of Arabidopsis. Since the first edition of Arabidopsis Protocols appeared, new developments have fostered an impressive advance in plant biology that prompted us to prepare Arabidopsis Protocols, Second Edition. Completion of the Arabidopsis genome sequence offered for the first time the opportunity to have in hand all of the genetic information required for studying plant function. In addition, the development of whole systems approaches that allow global analysis of gene expression and protein and metabolite dynamics has encouraged scientists to explore new scenarios that are extending the limits of our knowledge.

• DNA
• Embryo
• Genotyp
• Mutant
• Mutation
• PCR
• Seed
• arabidopsis thaliana
• biotechnology
• gene expression
• genes
• genetic engineering
• hybridization
• transcription

From the reviews of the first edition:

". . .a comprehensive collection of significant new methods as well as updated standard protocols. . .written by experienced authors and internationally recognized experts."-Journal of Plant Physiology

"...recommend[ed]...provides a set of enduring methods and is comprehensive enough for instant use in the lab" -Trends in Plant Science

"...a 'must have' for every laboratory involved in Arabibopsis" -Quarterly Review of Biology

"...a 'must' for those labs working with not just Arabidopsis specifically but plants in general..." -Microbiology Today

"...a must for anybody moving into the Arabidopsis field and setting up a new lab...extremely useful" -Genetical Research

"...especially valuable for detailed step-by-step procedures including many important notes...an indispensable guide, which should be at reach on the shelf in each laboratory where the plant molecular-genetic methods in Arabidopsis, and not only Arabidopsis, are used."- Biologia Plantarum

"...should prove to be highly helpful laboratory companion for beginners and established researchers in molecular biology, particularly those whose research deal with Arabidopsis and related plants." - Plant Science Bulletin

From the reviews of the second edition:

"Arabidopsis Protocols is another volume in Humana’s series, Methods in Molecular Biology. … It is … important to provide both experienced researchers and those new to the use of Arabidopsis, with single volumes that describe a wide range of experimental protocols. This book does that admirably. It is written by renowned experts in their respective fields … . Most chapters include useful Figures to illustrate the text. So I would heartily recommend this to any lab serious about carrying out work with Arabidopsis … ." (Keith Lindsey, Molecular Biotechnology, Vol. 36, 2007)

• Dept. of Biotechnology, Instituto Nacional de Investigacion y Tecnología Agraria y Alimentaria (INIA), Madrid, Spain

  Julio Salinas

• Dept. de Genetica Molecular de Plantas, Centro Nacional de Biotecnología-CSIC, Campus de Cantoblanco, Madrid, Spain

  Jose J. Sanchez-Serrano

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