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Breast Cancer Research Protocols

  • Book
  • © 2006

Overview

Editors:
  1. Susan A. Brooks

    1. School of Biological and Molecular Sciences, Oxford Brookes University, Headington, UK

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    You can also search for this editor in PubMed   Google Scholar

  2. Adrian Harris

    1. Medical Oncology Unit, The Churchill Hospital, Headington, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Medicine (MIMM, volume 120)

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Table of contents (35 protocols)

  1. Front Matter

    Pages i-xiv
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  2. Preparation of Cell and Tissue Samples

    1. Front Matter

      Pages 1-1
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    2. Breast Tissue Banking

      • Linda Snell, Peter H. Watson
      Pages 3-24

    3. Frozen Biopsy Collection and Storage

      • Russell Leek
      Pages 25-28

    4. Serial Biopsies/Fine-Needle Aspirates and Their Assessment

      • Irene Boeddinghaus, Stephen R. D. Johnson
      Pages 29-41

    5. Breast Tissue Microarrays

      • Robert J. Springall, ChlllLeryl E. Gillett
      Pages 43-50

    6. Preparation of Tumor Homogenates for Subsequent Preparation of Cytosols, Membrane Fractions, RNA, and DNA

      • Naomi Robertson, Russell Leek
      Pages 51-53

    7. Isolation of RNA From Tumor Samples

      • Naomi Robertson, Russell Leek
      Pages 55-59

    8. Isolation of DNA From Tumor Samples

      • Naomi Robertson, Russell Leek
      Pages 61-63

    9. Laser-Assisted Microdissection and Isolation of DNA and RNA

      • Ulrich Lehmann, Hans Kreipe
      Pages 65-75

  3. Markers of Clinical Outcome and Prognosis

    1. Front Matter

      Pages 77-77
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    2. Traditional and Established Indicators of Prognosis and Treatment Success

      • Derek E. Roskell, Ian D. Buley
      Pages 79-89

    3. Sentinel Lymph Node Biopsy in Breast Cancer

      • Celia Chao, Kelly M. McMasters
      Pages 91-111

    4. Pathological Evaluation of Axillary Sentinel Lymph Nodes in Breast Cancer

      • Michael Z. Gilcrease, Aysegul Sahin
      Pages 113-125

    5. Measurement of Estrogen Receptor Status by Immunocytochemistry in Paraffin Wax Sections

      • Bharat Jasani, Anthony Douglas-Jones, Anthony Rhodes, Susan Wozniak, Peter J. Barrett-Lee, Julie Gee et al.
      Pages 127-146

    6. Markers of Apoptosis

      • Fazlul H. Sarkar, Yiwei Li
      Pages 147-160

    7. Quantitative Angiogenesis in Breast Cancer

      • Stephen B. Fox
      Pages 161-187

  4. Analysis of Tumor-Derived Proteins and Antigens

    1. Front Matter

      Pages 189-189
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    2. Immunohistochemistry

      • Cheryl E. Gillett
      Pages 191-200

    3. Detection of Aberrant Glycosylation in Breast Cancer Using Lectin Histochemistry

      • Tracey M. Carter, Susan A. Brooks
      Pages 201-216
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Keywords

About this book

A collection of both well-established and cutting-edge methods for investigating breast cancer biology not only in the laboratory, but also in clinical settings. These readily reproducible techniques solve a variety of problems, ranging from how to collect, store, and prepare human breast tumor samples for analysis, to analyzing cells in vivo and in vitro. Additional chapters address the technology of handling biopsies, new methods for analyzing genes and gene expression, markers of clinical outcome and progress, analysis of tumor-derived proteins and antigens, validating targets, and investigating the biology of newly discovered genes.

Editors and Affiliations

  • School of Biological and Molecular Sciences, Oxford Brookes University, Headington, UK

    Susan A. Brooks

  • Medical Oncology Unit, The Churchill Hospital, Headington, UK

    Adrian Harris

Bibliographic Information

  • Book Title: Breast Cancer Research Protocols

  • Editors: Susan A. Brooks, Adrian Harris

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/1592599699

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2006

  • Hardcover ISBN: 978-1-58829-191-2Published: 01 November 2005

  • Softcover ISBN: 978-1-61737-413-5Published: 16 December 2010

  • eBook ISBN: 978-1-59259-969-1Published: 02 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: XIV, 518

  • Topics: Oncology

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