Multiple Myeloma

1. Front Matter

   Pages i-xi

   PDF

2. Multiple Myeloma

   • Douglas Joshua, Ross Brown, P. Joy Ho

   Pages 1-4

3. Immunophenotyping of Plasma Cells in Multiple Myeloma

   • Gema Mateo Manzanera, Jesús F. San Miguel Izquierdo, Alberto Orfao de Matos

   Pages 5-24

4. Plasma Cell Labeling Index

   • Philip R. Greipp, Shaji Kumar

   Pages 25-35

5. Conventional Cytogenetics in Myeloma

   • Lynda J. Campbell

   Pages 37-48

6. Multicolor Spectral Karyotyping in Multiple Myeloma

   • Jeffrey R. Sawyer

   Pages 49-58

7. Detection of Chromosome 13 Deletions by Fluorescent In Situ Hybridization

   • Scott VanWier, Rafael Fonseca

   Pages 59-69

8. Comparative Genomic Hybridization for Analysis of Changes in DNA Copy Number in Multiple Myeloma

   • Pulivarthi H. Rao

   Pages 71-83

9. Southern Blotting of IgH Rearrangements in B-Cell Disorders

   • Edna Yuen, Ross D. Brown

   Pages 85-103

10. Identification and Assembly of V Genes as Idiotype-Specific DNA Fusion Vaccines in Multiple Myeloma

    • Surinder S. Sahota, Mark Townsend, Freda K. Stevenson

    Pages 105-119

11. Identification of Clonotypic IgH VDJ Sequences in Multiple Myeloma

    • Brian J. Taylor, Jitra Kriangkum, Erin R. Strachan, Juanita Wizniak, Linda M. Pilarski

    Pages 121-144

12. Real-Time Polymerase Chain Reaction of Immunoglobulin Rearrangements for Quantitative Evaluation of Minimal Residual Disease in Myeloma

    • Mara Compagno, Barbara Mantoan, Monica Astolfi, Mario Boccadoro, Marco Ladetto

    Pages 145-163

13. Incomplete DJH Rearrangements

    • David González, Ramón Garcia-Sanz

    Pages 165-173

14. Identification of Malignant Plasma Cells by mRNA In Situ Hybridization

    • Ross D. Brown, P. Joy Ho

    Pages 175-181

15. The SCID-hu Myeloma Model

    • Joshua Epstein, Shmuel Yaccoby

    Pages 183-190

16. The 5T2MM Murine Model of Multiple Myeloma

    • Karin Vanderkerken, Kewal Asosingh, Angelo Willems, Hendrik de Raeve, Pedro Couck, Frans Gorus et al.

    Pages 191-205

17. Determination of Telomerase Activity and Telomere Length

    • Kai-da Wu, Malcolm A. S. Moore

    Pages 207-223

18. Dendritic Cell/Myeloma Hybrid Vaccine

    • Dajing Xia, Tim Chan, Jim Xiang

    Pages 225-234

19. Genetically Engineered Myeloma Cell Vaccine

    • Siguo Hao, Tim Chan, Jim Xiang

    Pages 235-244

20. An In Vitro Osteoclast-Forming Assay to Measure Myeloma Cell-Derived Osteoclast-Activating Factors

    • Andrew C. W. Zannettino, Amanda N. Farrugia, L. Bik To, Gerald J. Atkins

    Pages 245-256

A comprehensive collection of classic and innovative methodologies used in many laboratories for the investigation of multiple myeloma. These readily reproducible techniques range from the standard Plasma Cell Labeling Index methodology to a final chapter on making sense of microarrays, and include the full spectrum of cytogenetic and molecular diagnostic methods. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. These proven techniques are ideal for studying the pathogenesis of multiple myeloma and identifying new therapeutic targets.

• DNA
• cell
• cytogenetics
• genetics
• pathogenesis
• plasma

From the reviews:

"...extremely informative...a great reference for researchers or students with an interest in the etiology and treatment of Multiple Myeloma." - The Journal of the Association of Genetic Technologists

"Audience: Hematologists, oncologists, and other clinicians … as well as biomedical researchers in the field of hematology and immunology. … The book is divided into 23 chapters, including schematic diagrams, figures, color plates, and tables, as well as an index of important terms at the end. … covers clinical as well as experimental aspects of multiple myeloma and provides an important set of information for clinicians and researchers, in different fields of biomedicine, including hematology, oncology, molecular diagnostic, clinical immunology, osteoimmunology, molecular-biology, genetics, and bioinformatics.” (Danka Grcevic, Croatian Medical Journal, Vol. 48, October, 2007)

• Institute of Haematology, Royal Prince Alfred Hospital, Sydney, Australia

  Ross D. Brown, P. Joy Ho

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