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Peptide Synthesis and Applications

  • Book
  • © 2005

Overview

Editors:
  1. John Howl

    1. Research Institute in Healthcare Science, School of Applied Sciences, University of Wolverhampton, Wolverhampton, UK

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Part of the book series: Methods in Molecular Biology (MIMB, volume 298)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-x
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  2. Common Strategies

    1. Front Matter

      Pages 1-1
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    2. Fundamentals of Modern Peptide Synthesis

      • Muriel Amblard, Jean-Alain Fehrentz, Jean Martinez, Gilles Subra
      Pages 3-24

    3. Chimerism

      • John Howl
      Pages 25-41

  3. Synthetic Methodologies and Applications

    1. Front Matter

      Pages 43-43
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    2. Modification of Peptides and Other Drugs Using Lipoamino Acids and Sugars

      • Joanne T. Blanchfield, Istvan Toth
      Pages 45-61

    3. Synthesis of Linear, Branched, and Cyclic Peptide Chimera

      • Gábor Mezö, Ferenc Hudecz
      Pages 63-76

    4. Synthesis of Cell-Penetrating Peptides for Cargo Delivery

      • Margus Pooga, lo Langel
      Pages 77-89

    5. Incorporation of Phosphotyrosyl Mimetic 4- (Phosphonodifluoromethyl)phenylalanine (F2Pmp) Into Signal Transduction-Directed Peptides

      • Zhu-Jun Yao, Kyeong Lee, Terrence R. Burke Jr.
      Pages 91-103

    6. Expressed Protein Ligation for Protein Semisynthesis and Engineering

      • Zuzana Machova, Annette G. Beck-Sickinger
      Pages 105-130

    7. Cellular Delivery of Peptide Nucleic Acid by Cell-Penetrating Peptides

      • Kalle Kilk, lo Langel
      Pages 131-141

    8. Quenched Fluorescent Substrate- Based Peptidase Assays

      • Rebecca A. Lew, Nathalie Tochon-Danguy, Catherine A. Hamilton, Karen M. Stewart, Marie-Isabel Aguilar, A. Ian Smith
      Pages 143-150

    9. A Convenient Method for Synthesis of Cyclic Peptide Libraries

      • Gregory T. Bourne, Jonathon L. Nielson, Justin F. Coughlan, Paul Darwen, Marc R. Campitelli, Douglas A. Horton et al.
      Pages 151-166

    10. High-Throughput Peptide Synthesis

      • Michal Lebl, John Hachmann
      Pages 167-194

    11. Backbone Amide Linker Strategies for the Solid-Phase Synthesis of C - Terminal Modified Peptides

      • Jordi Alsina, Steven A. Kates, George Barany, Fernando Albericio
      Pages 195-208

    12. 13 Synthesis of Peptide Bioconjugates

      • Ferenc Hudecz
      Pages 209-223

  4. Practical Guides

    1. Front Matter

      Pages 225-225
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    2. Protein Identification by Mass Spectrometric Analyses of Peptides

      • Ashley Martin
      Pages 227-240

    3. Manual Solid Phase Synthesis of Glutathione Analogs

      • Ursel Soomets, Mihkel Zilmer, lo Langel
      Pages 241-257

  5. Back Matter

    Pages 259-262
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Keywords

About this book

Hands-on experts describe in step-by-step detail the key methodologies of contemporary peptide synthesis and illustrate their numerous applications. The techniques presented include protocols for chemical ligation, the synthesis of cyclic and phosphotyrosine-containing peptides, lipoamino acid- and sugar-conjugated peptides, and peptide purification and analyses. Additional chapters detail methodologies and instrumentation for high-throughput peptide synthesis, many different applications of peptides as novel research tools and biological probes, and the design and application of fluorescent substrate-based peptides that can be used to determine the selectivity and activity of peptidases. A practical guide to the identification of proteins using mass spectrometric analyses of peptide mixtures is also included.

Editors and Affiliations

  • Research Institute in Healthcare Science, School of Applied Sciences, University of Wolverhampton, Wolverhampton, UK

    John Howl

Bibliographic Information

  • Book Title: Peptide Synthesis and Applications

  • Editors: John Howl

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592598773

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2005

  • Hardcover ISBN: 978-1-58829-317-6Published: 28 February 2005

  • Softcover ISBN: 978-1-61737-490-6Published: 19 November 2010

  • eBook ISBN: 978-1-59259-877-9Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 262

  • Number of Illustrations: 69 b/w illustrations

  • Topics: Biochemistry, general

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