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Amyloid Proteins

  • Book
  • © 2005

Overview

Editors:
  1. Einar M. Sigurdsson

    1. Departments of Psychiatry and Pathology, New York University School of Medicine, New York, NY

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Part of the book series: Methods in Molecular Biology (MIMB, volume 299)

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Table of contents (27 protocols)

  1. Front Matter

    Pages i-xiv
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  2. In Vitro Assays

    1. Front Matter

      Pages 1-1
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    2. Preparation of Aggregate-Free,Low Molecular Weight Amyloidβ for Assembly and Toxicity Assays

      • Gal Bitan, David B. Teplow
      Pages 3-9

    3. Determination of Peptide Oligomerization State Using Rapid Photochemical Crosslinking

      • Sabrina S. Vollers, David B. Teplow, Gal Bitan
      Pages 11-18

    4. In Vitro Preparation of Prefibrillar Intermediates of Amyloid-β and α Synuclein

      • Hilal A. Lashuel, Dolors Grillo-Bosch
      Pages 19-33

    5. Purification of Recombinant Tau Protein and Preparation of Alzheimer-Paired Helical Filaments In Vitro

      • Stefan Barghorn, Jacek Biernat, Eckhard Mandelkow
      Pages 35-51

    6. Cyclic Amplification of Protein Misfolding and Aggregation

      • Paula Saá, Joaquín Castilla, Claudio Soto
      Pages 53-65

    7. X-Ray Diffraction Studies of Amyloid Structure

      • O. Sumner Makin, Louise C. Serpell
      Pages 67-80

    8. Molecular Electron Microscopy Approaches to Elucidating the Mechanisms of Protein Fibrillogenesis

      • Hilal A. Lashuel, Joseph S. Wall
      Pages 81-101

    9. Time -Lapse Atomic Force Microscopy in the Characterization of Amyloid-Like Fibril Assembly and Oligomeric Intermediates

      • Claire Goldsbury, Janelle Green
      Pages 103-128

    10. Fourier Transform Infrared and Circular Dichroism Spectroscopies for Amyloid Studies

      • Miguel Calero, María Gasset
      Pages 129-151

    11. Quasielastic Light Scattering for Protein Assembly Studies

      • Aleksey Lomakin, David B. Teplow, George B. Benedek
      Pages 153-174

    12. Intrinsic Fluorescent Detection of Tau Conformation and Aggregation

      • Martin von Bergen, Li Li, Eckhard Mandelkow
      Pages 175-184

    13. Quantitative Measurement of Fibrillogenesis by Mass Spectrometry

      • Andrew D. Miranker
      Pages 185-194

  3. Cell Culture Assays

    1. Front Matter

      Pages 195-195
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    2. Isolation and Culturing of Human Vascular Smooth Muscle Cells

      • Finnbogi R. Thormodsson, Ingvar H. Olafsson
      Pages 197-210

    3. Murine Cerebrovascular Cells as a Cell Culture Model for Cerebral Amyloid Angiopathy

      • Sonia S. Jung, Efrat Levy
      Pages 211-219

    4. Purification of Human Wild-Type or Variant Cystatin C From Conditioned Media of Transfected Cells

      • Frances Prelli, Monika Pawlik, Blas Frangione, Efrat Levy
      Pages 221-226

    5. Prion Propagation in Cell Culture

      • Sylvain Lehmann
      Pages 227-234

  4. In Vivo-Related Assays

    1. Front Matter

      Pages 235-235
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Keywords

About this book

A proven collection of readily reproducible techniques for studying amyloid proteins and their involvement in the etiology, pathogenesis, diagnosis, and therapy of amyloid diseases. The contributors provide methods for the preparation of amyloid and its precursors (oligomers and protofibrils), in vitro assays and analytical techniques for their study, and cell culture models and assays for the production of amyloid proteins. Additional chapters present readily reproducible techniques for amyloid extraction from tissue, its detection in vitro and in vivo, as well as nontransgenic methods for developing amyloid mouse models. The protocols follow the successful Methods in Molecular Biologyâ„¢ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Editors and Affiliations

  • Departments of Psychiatry and Pathology, New York University School of Medicine, New York, NY

    Einar M. Sigurdsson

Bibliographic Information

  • Book Title: Amyloid Proteins

  • Editors: Einar M. Sigurdsson

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592598749

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2005

  • Softcover ISBN: 978-1-61737-502-6Published: 19 November 2010

  • eBook ISBN: 978-1-59259-874-8Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: 408

  • Number of Illustrations: 155 b/w illustrations, 1 illustrations in colour

  • Topics: Biochemistry, general

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