Molecular Toxicology Protocols

1. Front Matter

   Pages i-xiv

   PDF

2. Analysis of DNA Adducts

   1. Front Matter

      Pages 1-1

      PDF

   2. 32P-Postlabeling Analysis of DNA Adducts

      • David H. Phillips, Alan Hewer, Volker M. Arlt

      Pages 3-12

   3. Modification of the 32P-Postlabeling Method to Detect a Single Adduct Species as a Single Spot

      • Masako Ochiai, Takashi Sugimura, Minako Nagao

      Pages 13-19

   4. DNA Isolation and Sample Preparation for Quantification of Adduct Levels by Accelerator Mass Spectrometry

      • Karen H. Dingley, Esther A. Ubick, John S. Vogel, Kurt W. Haack

      Pages 21-27

   5. Fluoroimaging-Based Immunoassay of DNA Photoproducts in Ultraviolet-B-Irradiated Tadpoles

      • Iovanna Pandelova, Stephen R. Hewitt, John B. Hays

      Pages 29-38

   6. Analysis of DNA Strand Cleavage at Abasic Sites

      • Walter A. Deutsch, Vijay Hegde

      Pages 39-46

3. Detection of Chromosomal and Genome-Wide Damage

   1. Front Matter

      Pages 47-47

      PDF

   2. Premature Chromosome Condensation in Human Resting Peripheral Blood Lymphocytes for Chromosome Aberration Analysis Using Specific Whole-Chromosome DNA Hybridization Probes

      • Pataje G. S. Prasanna, William F. Blakely

      Pages 49-57

   3. Mutagen-Induced Chromatid Breakage as a Marker of Cancer Risk

      • Xifeng Wu, Yun-Ling Zheng, T. C. Hsu

      Pages 59-67

   4. Flow Cytometric Analysis of Micronuclei in Erythrocytes

      • Jan Grawé

      Pages 69-83

   5. The Comet Assay

      • Günter Speit, Andreas Hartmann

      Pages 85-95

   6. Computerized Image Analysis Software for the Comet Assay

      • R. C. Chaubey

      Pages 97-106

   7. The Comet-FISH Technique

      • Alexander Rapp, Michael Hausmann, Karl Otto Greulich

      Pages 107-119

   8. DNA Double-Strand Break Damage and Repair Assessed by Pulsed-Field Gel Electrophoresis

      • Nina Joshi, Stephen G. Grant

      Pages 121-129

4. Detection and Characterization of Surrogate Gene Mutation

   1. Front Matter

      Pages 131-131

      PDF

   2. Analysis of In Vivo Mutation in the Hprt and Tk Genes of Mouse Lymphocytes

      • Vasily N. Dobrovolsky, Joseph G. Shaddock, Robert H. Heflich

      Pages 133-144

   3. Quantifying In Vivo Somatic Mutations Using Transgenic Mouse Model Systems

      • Roy R. Swiger

      Pages 145-153

   4. Methods for Detecting Somatic Mutations In Vitro

      • Sai-Mei Hou

      Pages 155-160

   5. Molecular Analysis of Mutations in the Human HPRT Gene

      • Phouthone Keohavong, Liqiang Xi, Stephen G. Grant

      Pages 161-170

A collection of cutting-edge techniques for analyzing genotoxic exposure and detecting the resulting biological effects-including endogenous metabolites-up to and including the development of cancer. The authors emphasize analytical methods that can be specifically applied to human populations and patients. Among the applications detailed are the analysis of interactions between such cellular macromolecules as DNA and proteins and chemical and physical agents, the assessment of medically relevant toxicity, and the characterization of genetic alterations induced in transgenic animals by in vivo systems. There are also methods for the analysis of genotoxic exposure during gene expression, of cytotoxicity caused by the induction of apoptosis, of genetic alterations in reporter genes and oncogenes, early (premalignant) detection of altered oncogenes, and of individual variation in biotransformation and DNA repair capacity.

• Allele
• DNA
• FISH
• Ligation
• PCR
• cancer risk
• chromium
• gene expression
• genes
• hybridization
• toxicology
• transgenic animals

"This book should be of interest to both specialist researchers as well as members of the wider scientific community..." - British Journal of Anesthesiology

• Department of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh

  Phouthone Keohavong, Stephen G. Grant

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