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Developmental Hematopoiesis

Methods and Protocols

  • Book
  • © 2005

Overview

Editors:
  1. Margaret H. Baron

    1. Department of Medicine (Hematology-Oncology), Mount Sinai School of Medicine, New York

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Medicine (MIMM, volume 105)

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Table of contents (29 protocols)

  1. Front Matter

    Pages i-xv
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  2. Genetic Approaches

    1. Front Matter

      Pages 1-1
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    2. Gene Targeting and Transgenic Strategies for the Analysis of Hematopoietic Development in the Mouse

      • Hanna K. A. Mikkola, Stuart H. Orkin
      Pages 3-21

    3. Inducible Transgene Expression in Mouse Stem Cells

      • David T. Ting, Michael Kyba, George Q. Daley
      Pages 23-46

    4. Quantitative Trait Analysis in the Investigation of Function and Aging of Hematopoietic Stem Cells

      • Hans-Willem Snoeck
      Pages 47-62

  3. Analysis of Cell Fate Outcomes in Transplantation Models

    1. Front Matter

      Pages 63-63
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    2. Flow Cytometric Analysis of Hematopoietic Development

      • Edward F. Srour, Mervin C. Yoder
      Pages 65-79

    3. Reconstitution of Hematopoiesis Following Intrauterine Transplantation of Stem Cells

      • Elisabeth H. Javazon, Aziz M. Merchant, Enrico Danzer, Alan W. Flake
      Pages 81-94

    4. Reconstitution of Hematopoiesis Following Transplantation Into Neonatal Mice

      • Scott A. Johnson, Mervin C. Yoder
      Pages 95-105

  4. Animal Models

    1. Front Matter

      Pages 107-107
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    2. Drosophila

      1. Hemocyte Development During <i>Drosophila</i> Embryogenesis
        • Robert A. Schulz, Nancy Fossett
        Pages 109-121

    4. Zebrafish

      1. Analysis of Hematopoietic Development in the Zebrafish
        • Noëlle N. Paffett-Lugassy, Leonard I. Zon
        Pages 171-198
      2. Imaging Early Macrophage Differentiation, Migration, and Behaviors in Live Zebrafish Embryos
        • Philippe Herbomel, Jean-Pierre Levraud
        Pages 199-214
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Keywords

About this book

During the past few decades, technical and conceptual breakthroughs have led to a virtual revolution in developmental biology. In part through cross-species compa- sons and multidisciplinary approaches (combining, for example, classical embry- ogy, genetics, molecular biology, and systems biology), major questions have often been redefined and examined from new angles and with innovative tools. Analyses using such model systems as Drosophila, Xenopus, zebrafish, chick, human, and mouse have underscored the remarkable extent to which molecular and genetic pa- ways are conserved across species and throughout embryonic, fetal, and adult dev- opment. What we learn from the embryo, then, is not only of fundamental interest, but may well have future practical applications in the clinic. A number of excellent volumes, including several in this series (e. g. , Hema- poietic Stem Cell Protocols, Klug and Jordan, eds. , 2002), have surveyed methods used in the study of hematopoiesis—the processes by which the multiple lineages of the blood form from stem and progenitor cells during ontogeny and throughout the entire life of the animal. These collections of protocols have focused largely on the postnatal cells of mouse and human. Our understanding of hematopoietic devel- ment, however, has benefitted enormously from investigations in a variety of org- isms at different stages of ontogeny.

Editors and Affiliations

  • Department of Medicine (Hematology-Oncology), Mount Sinai School of Medicine, New York

    Margaret H. Baron

Bibliographic Information

  • Book Title: Developmental Hematopoiesis

  • Book Subtitle: Methods and Protocols

  • Editors: Margaret H. Baron

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/1592598269

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2005

  • Hardcover ISBN: 978-1-58829-296-4Published: 15 October 2004

  • Softcover ISBN: 978-1-61737-478-4Published: 10 November 2010

  • eBook ISBN: 978-1-59259-826-7Published: 24 April 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: 480

  • Number of Illustrations: 92 b/w illustrations, 4 illustrations in colour

  • Topics: Hematology

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