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  • Book
  • © 2004

Protein'Protein Interactions

Methods and Applications

Editors:

  1. Haian Fu

    1. Department of Pharmacology, Emory University School of Medicine, Atlanta

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 261)

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Table of contents (35 protocols)

  1. Front Matter

    Pages i-xvi
    PDF

  2. Overview

    1. Front Matter

      Pages 1-1
      PDF

    2. Structural Basis of Protein-Protein Interactions

      • Robert C. Liddington
      Pages 3-14

  3. In Vitro Techniques

    1. Front Matter

      Pages 33-33
      PDF

    2. Characterization of Protein-Protein Interactions by Isothermal Titration Calorimetry

      • Adrian Velazquez-Campoy, Stephanie A. Leavitt, Ernesto Freire
      Pages 35-54

    4. Protein-Protein Interaction Analysis by Nuclear Magnetic Resonance Spectroscopy

      • Guanghua Gao, Jason G. Williams, Sharon L. Campbell
      Pages 79-91

    7. Sedimentation Equilibrium Studies

      • Ian A. Taylor, John F. Eccleston, Katrin Rittinger
      Pages 119-136

    8. Analysis of Protein-Protein Interactions by Simulation of Small-Zone Gel Filtration Chromatography

      • Rosemarie Wilton, Elizabeth A. Myatt, Fred J. Stevens
      Pages 137-154

    9. Fluorescence Gel Retardation Assay to Detect Protein-Protein Interactions

      • Sang-Hyun Park, Ronald T. Raines
      Pages 155-159

    10. Fluorescence Polarization Assay to Quantify Protein-Protein Interactions

      • Sang-Hyun Park, Ronald T. Raines
      Pages 161-165

  4. Detecting Protein-Protein Interactions in Heterologous Systems

    1. Front Matter

      Pages 229-229
      PDF
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About this book

As the mysteries stored in our DNA have been more completely revealed, scientists have begun to face the extraordinary challenge of unraveling the int- cate network of protein–protein interactions established by that DNA fra- work. It is increasingly clear that proteins continuously interact with one another in a highly regulated fashion to determine cell fate, such as proliferation, diff- entiation, or death. These protein–protein interactions enable and exert str- gent control over DNA replication, RNA transcription, protein translation, macromolecular assembly and degradation, and signal transduction; essentially all cellular functions involve protein–protein interactions. Thus, protein–p- tein interactions are fundamental for normal physiology in all organisms. Alt- ation of critical protein–protein interactions is thought to be involved in the development of many diseases, such as neurodegenerative disorders, cancers, and infectious diseases. Therefore, examination of when and how protein–p- tein interactions occur and how they are controlled is essential for understa- ing diverse biological processes as well as for elucidating the molecular basis of diseases and identifying potential targets for therapeutic interventions. Over the years, many innovative biochemical, biophysical, genetic, and computational approaches have been developed to detect and analyze p- tein–protein interactions. This multitude of techniques is mandated by the diversity of physical and chemical properties of proteins and the sensitivity of protein–protein interactions to cellular conditions.
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Reviews

From the reviews:

"This book is thorough and packed with information...the volume is of high practical value and is fully recommended to all biochemists..." - Cell Biochemistry and Function

"The book is well-balanced and easy to use...well worth the purchasing cost." - ChemBioChem

"This … volume comprehensively describes a variety of biophysical-, cell biology- and molecular biology-based approaches to the analysis of protein-protein interactions. … This volume is recommended as a solid presentation on the fundamentals of protein interaction analysis, suitable for graduate biophysical chemistry courses and for those investigators new to the field who would like to get a solid grounding … ." (Wayne F. Patton, Proteomics, Issue 6, 2006)

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Editors and Affiliations

  • Department of Pharmacology, Emory University School of Medicine, Atlanta

    Haian Fu

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Bibliographic Information

  • Book Title: Protein'Protein Interactions

  • Book Subtitle: Methods and Applications

  • Editors: Haian Fu

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592597629

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2004

  • Hardcover ISBN: 978-1-58829-120-2Published: 23 March 2004

  • Softcover ISBN: 978-1-61737-373-2Published: 10 November 2010

  • eBook ISBN: 978-1-59259-762-8Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 532

  • Topics: Biochemistry, general

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Buy it now

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

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