Overview
- Editors:
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Marie-Isabel Aguilar
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Department of Biochemistry and Molecular Biology, Monash University, Clayton, Australia
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Table of contents (27 protocols)
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Front Matter
Pages i-xiii
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Modes of HPLC
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- Herbert Lindner, Wilfried Helliger
Pages 75-88
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- Tun-Li Shen, Kathleen R. Noon
Pages 111-139
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Preparative Methodologies
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Front Matter
Pages 141-141
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- Montserrat Carrascal, Joaquin Abian
Pages 143-164
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- Edouard C. Nice, Marie-Isabel Aguilar
Pages 165-175
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- Edouard C. Nice, Marie-Isabel Aguilar
Pages 177-182
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- Denis B. Scanlon, James Finlayson
Pages 191-209
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- Joseph Bertolini, Peter Gomme, Patrick Thomas
Pages 211-223
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Applications
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Front Matter
Pages 225-225
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About this book
The introduction of high-performance liquid chromatography (HPLC) to the analysis of peptides and proteins some 25 years ago revolutionized the biological sciences by enabling the rapid and sensitive analysis of peptide and protein structure through the exquisite speed, sensitivity, and resolution that can be easily obtained. Today, HPLC in its various modes has become the pivotal technique in the characterization of peptides and proteins and currently plays a critical role in both our understanding of biological processes and in the development of peptide- and protein-based pharmaceuticals. The number of applications of HPLC in peptide and protein purification continues to expand at an extremely rapid rate. Solid-phase peptide synthesis and recombinant DNA techniques have allowed the production of large quantities of peptides and proteins that need to be highly purified. HPLC techniques are also used extensively in the isolation and characterization of novel proteins that will become increasingly important in the postgenomic age. The design of multidimensional purification schemes to achieve high levels of product purity further demonstrates the power of HPLC techniques not only in the characterization of cellular events, but also in the production of pepti- and protein-based therapeutics. HPLC continues to be at the heart of the analytical techniques with which scientists in both academia and in industry must arm themselves to be able to fully characterize the identity, purity, and potency of peptides and proteins.
Reviews
"...a showcase of state-of-the-art methodologies in the application of HPLC to protein/peptide studies...can be used by beginners and intermediate experts in this field to develop separation and analytical protocols...all scientists dealing with peptides and proteins can benefit from reading this book." - Proteomics
"...this book is well written...a very usable laboratory companion or reference guide for researchers in pursuit of peptide and protein characterizations." - Biomedical Chromatography
Editors and Affiliations
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Department of Biochemistry and Molecular Biology, Monash University, Clayton, Australia
Marie-Isabel Aguilar
