Lipoprotein Protocols

1. Front Matter

   Pages i-x

   PDF

2. DNA- and RNA-Based Methodolog

   1. PCR Methodology Applied to Genetic Studies of Lipoprotein Metabolism and Atherosclerosis

      • Shelley A. Cole, James E. Hixson

      Pages 1-34

   2. Separation of Small-Size DNA Fragments Using Agarose Gel Electrophoresis

      • Jose M. Ordovas

      Pages 35-42

   3. Quantification of mRNA by Polymerase Chain Reaction (PCR) Using an Internal Standard and a Nonradioactive Detection Method

      • William H. Karge III, Ernst J. Schaefer, Jose M. Ordovas

      Pages 43-61

   4. Generation of Transgenic Mice

      • Marten H. Hofker, Marco L. Breuer

      Pages 63-78

   5. Preparation of Knockout Mice

      • Jesús Osada, Nobuyo Maeda

      Pages 79-92

3. Protein-Based Methodology

   1. Fast Ultracentrifugation Methods for the Separation of Plasma Lipoproteins

      • Jose M. Ordovas

      Pages 93-103

   2. Preparative Isolation of Plasma Lipoproteins Using Fast Protein Liquid Chromatography (FPLC)

      • Jose M. Ordovas, Doreen Osgood

      Pages 105-111

   3. Separation of Apolipoproteins by Polyacrylamide Gel Electrophoresis

      • Jose M. Ordovas

      Pages 113-130

   4. Isolation and Purification of Serum Lipoprotein (a) Using Lectin Affinity

      • Leo J. Seman, Carl de Luca, Jennifer L. Jenner

      Pages 131-136

   5. Electrophoretic Separation of LDL and HDL Subclasses

      • David L. Rainwater

      Pages 137-151

   6. Measurement of Lipoprotein Particles

      • Catherine Fievet, Jean-Charles Fruchart

      Pages 153-166

   7. Methodological Approaches for Assessing Lipid and Protein Oxidation and Modification in Plasma and Isolated Lipoproteins

      • Wolfgang Sattler, Ernst Malle, Gert M. Kostner

      Pages 167-191

   8. Separation and Quantitation of Phospholipid Classes by HPLC

      • George M. Patton, Sander J. Robins

      Pages 193-215

   9. Assays of Lecithin Cholesterol Acyltransferase (LCAT)

      • Milada Dobiásová, Jiri J. Frohlich

      Pages 217-230

   10. Determination of the Mass Concentration and the Activity of the Plasma Cholesteryl Ester Transfer Protein (CETP)

       • Laurent Lagrost

       Pages 231-241

   11. In Vitro Measurement of Lipoprotein and Hepatic Lipases

       • Elisabet Vilella, Jorge Joven

       Pages 243-251

   12. Cellular Assays for Lipoprotein Receptors

       • Susan Acton, Attilio Rigotti

       Pages 253-266

   13. Measurements of Proteoglycan—Lipoprotein Interaction by Gel Mobility Shift Assay

       • Eva Hurt-Camejo, Germán Camejo, Peter Sartipy

       Pages 267-279

4. Back Matter

   Pages 281-284

   PDF

In Lipoprotein Protocols, Jose Ordovas compiles a cutting-edge collection of molecular protein techniques for studying advanced aspects of lipoprotein structure and metabolism. These step-by-step methods allow both the beginning and the advanced researcher to carry out not only fundamental, but also the most advanced genetic screening techniques, including RT-PCR and the preparation of relevant models of transgenic and knockout mice. In addition, novel techniques are presented to characterize plasma lipoproteins, their subfractions, and their modifications, and to determine the activity of relevant enzymes and carrier proteins. Each method is described in easily reproducible detail by hands-on experts who have perfected it.

Lipoprotein Protocols offers many powerful tools for unraveling the complexity of lipoprotein metabolism and determining the individual risk of coronary heart disease. For
all those basic and clinical investigators seeking to advance the frontier of lipoprotein research and develop novel genetic therapies for the treatment of cardiovascular disease, this eminently useful book constitutes the new standard reference and guide.

"This text makes a good selection with admirable breadth and a high general quality of method description."-Clin Chem Lab Med

"The book presents the state-of-art of molecular biology and protein techniques specific for lipoprotein research...The book may be especially recommended for students who start working on lipoproteins, but may also be generally helpful for molecular biologists..."-Nahrung

"Each chapter contains, besides a description of the principle of the methods, highly instructive protocols and lists of the technical requirements and reagents. Notes with additional information, e.g. special experimental conditions or safety and technical advises as well as numerous references are given. The book may be especially recommended for students who start working on lipoproteins, but may also be generally helpful for molecular biologists."-Nahrung

• Tufts University, Boston

  Jose M. Ordovas

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