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E. coli

Shiga Toxin Methods and Protocols

  • Book
  • © 2003

Overview

Editors:
  1. Dana Philpott

    1. Groupe d&'Immunité Innée et Signalisation, Institut Pasteur, Paris, France

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    You can also search for this editor in PubMed   Google Scholar

  2. Frank Ebel

    1. Max-von-Pettenkofer-Institut, Bakteriologie, Munich, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Medicine (MIMM, volume 73)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. The Medical Significance of Shiga Toxin-Producing Escherichia coli Infections

    • Mohamed A. Karmali
    Pages 1-7

  3. Methods for Detection of STEC in Humans

    • James C. Paton, Adrienne W. Paton
    Pages 9-26

  4. Serological Methods for the Detection of STEC Infections

    • Martin Bitzan, Helge Karch
    Pages 27-43

  5. Detection and Characterization of STEC in Stool Samples Using PCR

    • Adrienne W. Paton, James C. Paton
    Pages 45-54

  6. Molecular Typing Methods for STEC

    • Haruo Watanabe, Jun Terajima, Hidemasa Izumiya, Sunao Iyoda
    Pages 55-65

  7. STEC in the Food Chain

    • Michael Bülte
    Pages 67-74

  8. STEC as a Veterinary Problem

    • Lothar H. Wieler, Rolf Bauerfeind
    Pages 75-89

  9. Cellular Microbiology of STEC Infections

    • Frank Ebel, Dana Philpott
    Pages 91-98

  10. Analysis of Pathogenicity Islands of STEC

    • Tobias A. Oelschlaeger, Ulrich Dobrindt, Britta Janke, Barbara Middendorf, Helge Karch, Jörg Hacker
    Pages 99-112

  11. Generation of Isogenic Deletion Mutants of STEC

    • Soudabeh Djafari, Nadja D. Hauf, Judith F. Tyczka
    Pages 113-124

  12. Generation of Monoclonal Antibodies Against Secreted Proteins of STEC

    • Kirsten Niebuhr, Frank Ebel
    Pages 125-135

  13. Microscopic Methods to Study STEC

    • Stuart Knutton
    Pages 137-149

  14. Detection and Characterization of EHEC-Hemolysin

    • Herbert Schmidt, Roland Benz
    Pages 151-163

  15. Shiga Toxin Receptor Glycolipid Binding

    • Clifford A. Lingwood
    Pages 165-186

  16. Methods for the Purification of Shiga Toxin 1

    • Anita Nutikka, Beth Binnington-Boyd, Clifford A. Lingwood
    Pages 187-195

  17. Methods for the Identification of Host Receptors for Shiga Toxin

    • Anita Nutikka, Beth Binnington-Boyd, Clifford A. Lingwood
    Pages 197-208

  18. Shiga Toxin B-Subunit as a Tool to Study Retrograde Transport

    • Frédéric Mallard, Ludger Johannes
    Pages 209-220

  19. Measuring pH Within the Golgi Complex and Endoplasmic Reticulum Using Shiga Toxin

    • Jae H. Kim
    Pages 221-228

  20. Detection of Shiga Toxin-Mediated Programmed Cell Death and Delineation of Death-Signaling Pathways

    • Nicola L. Jones
    Pages 229-241
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About this book

The study of the pathogenesis of Shiga toxin-producing Escherichia coli (STEC) infections encompasses many different disciplines, including clinical microbiology, diagnostics, animal ecology, and food safety, as well as the cellular microbiology of both bacterial pathogenesis and the mechanisms of toxin action. E. coli: Shiga Toxin Methods and Protocols aims to bring together a number of experts from each of these varied fields in order to o- line some of the basic protocols for the diagnosis and study of STEC pat- genesis. We hope that our book will prove a valuable resource for the clinical microbiologist as well as the cellular microbiologist. For the clinical microbiologist, our aim is to detail a number of current protocols for the detection of STEC in patient samples, each of which have their own advantages. Chapter 1 provides an introduction into the medical significance of STEC infections. Chapters 2–7 follow with protocols for the diagnosis and detection of STEC bacteria in patient and animal samples.

Reviews

"The authors should be thanked for providing this valuable resource tool that is highly recommended to microbiologists, molecular biologists and to all who are interested in bacterial toxin action. All medical libraries should house a copy of this text. The book also will serve as an excellent teaching guide for students and fellows particularly in postgraduate programs." -Folia Microbiologica

Editors and Affiliations

  • Groupe d&'Immunité Innée et Signalisation, Institut Pasteur, Paris, France

    Dana Philpott

  • Max-von-Pettenkofer-Institut, Bakteriologie, Munich, Germany

    Frank Ebel

Bibliographic Information

  • Book Title: E. coli

  • Book Subtitle: Shiga Toxin Methods and Protocols

  • Editors: Dana Philpott, Frank Ebel

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/159259316X

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2003

  • Hardcover ISBN: 978-0-89603-939-1Published: 26 September 2002

  • Softcover ISBN: 978-1-61737-264-3Published: 08 October 2011

  • eBook ISBN: 978-1-59259-316-3Published: 02 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: XII, 352

  • Topics: Microbiology

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