Overview
- Editors:
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Yao-Shan Fan
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London Health Sciences Center, London
The University of Western Ontario, Ontario, Canada
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Table of contents (27 protocols)
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Basic Concepts and Techniques
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- Larry E. Morrison, Ramesh Ramakrishnan, Teresa M. Ruffalo, Kim A. Wilber
Pages 21-40
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- Ramesh Ramakrishnan, Larry E. Morrison
Pages 41-49
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- Sue Van Stedum, Walter King
Pages 51-63
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Evolving Techniques and Applications
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- Jane Bayani, Jeremy A. Squire
Pages 85-104
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- Syed M. Jalal, Mark E. Law
Pages 105-120
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- Brynn Levy, Kurt Hirschhorn
Pages 121-132
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- Vicki L. Hopwood, Christi J. Hada, Armand B. Glassman
Pages 133-142
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- Jaennelle Kraan, Anne R. M. von Bergh, Karin Kleiverda, Jan-Willem Vaandrager, Ekaterina S. Jordanova, Anton K. Raap et al.
Pages 143-153
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- Samantha J. L. Knight, Jonathan Flint
Pages 155-179
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- Laurence Colleaux, Solange Heuertz, Florence Molinari, Marlene Rio
Pages 181-189
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- Ben Beheshti, Paul C. Park, Ilan Braude, Jeremy A. Squire
Pages 191-207
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Special Applications in Chromosomal Disorders
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Front Matter
Pages 209-209
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- Michael D. Graf, Stuart Schwartz
Pages 211-218
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- Baruch Feldman, Ayala Aviram-Goldring, Mark I. Evans
Pages 219-241
About this book
The new techniques of molecular cytogenetics, mainly fluorescence in situ hybridization (FISH) of DNA probes to metaphase chromosomes or interphase nuclei, have been developed in the past two decades. Many FISH techniques have been implemented for diagnostic services, whereas some others are mainly used for investigational purposes. Several hundreds of FISH probes and hybridization kits are now commercially available, and the list is growing rapidly. FISH has been widely used as a powerful diagnostic tool in many areas of medicine including pediatrics, medical genetics, maternal–fetal medicine, reproductive medicine, pathology, hematology, and oncology. Frequently, a physician may be puzzled by the variety of FISH techniques and wonder what test to order. It is not uncommon that a sample is referred to a laboratory for FISH without indicating a specific test. On the other hand, a cytogeneticist or a technologist in a laboratory needs, from case to case, to determine which procedure to perform and which probe to use for an informative result. To obtain the best results, one must use the right DNA probes and have reliable protocols and measures of quality assurance in place. Also, one must have sufficient knowledge in both traditional and molecular cytogenetics, as well as the particular areas of medicine for which the test is used in order to appropriately interpret the FISH results, and to correlate them with clinical diagnosis, treatment, and prognosis.
Reviews
"Dr. Fan has done a marvelous job of putting together a collection of laboratory protocols and review articles of different FISH technologies...Molecular Cytogenetics: Protocols and Applications is packed with information on cutting-edge FISH technologies and their applications, limitations, and pitfalls, along with detailed laboratory protocols. I would certainly recommend that this book be included in the libraries of cytogeneticists and cytogenetic laboratories" - Am. J. Hum. Gent.
"Dr. Fan has done a marvelous job of putting together a collection of laboratory protocols and review articles of different FISH technologies. The authors of the different chapters not only gave detailed, easy-to-follow protocols, but also provided their first-hand experiences of troubleshooting. Molecular Cytogenetics: Protocols and Applications is packed with information on cutting-edge FISH technologies and their applications, limitations, and pitfalls, along with detailed laboratory protocols. I would certainly recommend that this book be included in the libraries of cytogeneticists and cytogenetic laboratories." -The American Society of Human Genetics
Editors and Affiliations
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London Health Sciences Center, London
Yao-Shan Fan
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The University of Western Ontario, Ontario, Canada
Yao-Shan Fan
