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PCR Mutation Detection Protocols

  • Book
  • © 2002

Overview

Editors:
  1. Bimal D. M. Theophilus

    1. Department of Haematology, Birmingham Children’s Hospital NHS Trust, Birmingham, UK

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    You can also search for this editor in PubMed   Google Scholar

  2. Ralph Rapley

    1. Department of Biosciences, University of Hertfordshire, Hatfield, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 187)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Agarose and Polyacrylamide Gel Electrophoresis

    • Andrea M. Guilliatt
    Pages 1-12

  3. Internal Labeling of DNA Probes

    • Ralph Rapley, Bimal D. M. Theophilus
    Pages 13-15

  4. End-Labeling of DNA Probes

    • Adrian J. Harwood
    Pages 17-22

  5. Southern Blotting of Agarose Gels by Capillary Transfer

    • Ralph Rapley, Ian J. Williams
    Pages 23-27

  6. Restriction Fragment Length Polymorphism

    • Mohammad S. Enayat
    Pages 29-35

  7. PCR

    • Nicola Louise Jones
    Pages 37-46

  8. Allele-Specific Oligonucleotide PCR

    • Elaine K. Green
    Pages 47-50

  9. Long-Range PCR

    • Peter A. Davies, George Gray
    Pages 51-55

  10. Analysis of Nucleotide Sequence Variations by Solid-Phase Minisequencing

    • Anu Wartiovaara, Ann-Christine Syvänen
    Pages 57-63

  11. Cycle Sequencing of PCR Products

    • G. K. Surdhar
    Pages 65-72

  12. Fluorescent In Situ Hybridization

    • Sara A. Dyer, Elaine K. Green
    Pages 73-86

  13. The Protein Truncation Test

    • Carol A. Hardy
    Pages 87-108

  14. Mutation Detection in Factor VIII cDNA from Lymphocytes of Hemophilia A Patients by Solid Phase Fluorescent Chemical Cleavage of Mismatch

    • Naushin H. Waseem, Richard Bagnall, Peter M. Green, Francesco Giannelli
    Pages 109-124

  15. Denaturing Gradient Gel Electrophoresis

    • Yvonne Wallis
    Pages 125-135

  16. Conformation-Sensitive Gel Electrophoresis

    • Ian J. Williams, Anne C. Goodeve
    Pages 137-150

  17. SSCP/Heteroduplex Analysis

    • Andrew J. Wallace
    Pages 151-163

  18. Cleavase® Fragment Length Polymorphism Analysis for Genotyping and Mutation Detection

    • Laura Heisler, Chao-Hung Lee
    Pages 165-178

  19. Automated Genotyping Using the DNA MassArray™ Technology

    • Christian Jurinke, Dirk van den Boom, Charles R. Cantor, Hubert Köster
    Pages 179-192

  20. An Introduction to Bioinformatics

    • Henry Brzeski
    Pages 193-208
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About this book

1Bimal D. Theophilus and Ralph Rapley provide biological and clinical investigators with a comprehensive collection of new, recent, and updated PCR-based screening methods suitable for detecting the presence of both known and novel mutations. The methods cover point mutations (e.g., ASO-PCR, SSCP, DGGE, chemical cleavage), deletions (multiplex PCR, FISH, blotting), non-sense mutations (PTT), and more. The new and exciting techniques of DNA array analysis, along with such recently developed experimental methods as conformation-sensitive gel electrophoresis, are also included. Each chapter explains the basic theory behind the technique and provides valuable notes essential for its successful execution.

Reviews

"The book provides access to the highly productive and readily reproducible PCR-based mutation detection methods, that are behind many of today's major scientific and medical advances." - Clinical Laboratory International

Editors and Affiliations

  • Department of Haematology, Birmingham Children’s Hospital NHS Trust, Birmingham, UK

    Bimal D. M. Theophilus

  • Department of Biosciences, University of Hertfordshire, Hatfield, UK

    Ralph Rapley

Bibliographic Information

  • Book Title: PCR Mutation Detection Protocols

  • Editors: Bimal D. M. Theophilus, Ralph Rapley

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592592732

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2002

  • eBook ISBN: 978-1-59259-273-9Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 214

  • Topics: Cell Biology

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