Eicosanoid Protocols

1. Front Matter

   Pages i-xi

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2. Use of a Retroviral Vector for Expression of a Foreign Gene

   • Brent W. Miller, Aubrey R. Morrison

   Pages 1-9

3. Extraction of Eicosanoids from Biological Fluids, Cells, and Tissues

   • William S. Powell

   Pages 11-24

4. Assessment of Cyclooxygenase RNA Expression by Northern Hybridization

   • Zhonghong Guan, Aubrey R. Morrison

   Pages 25-33

5. Immunohistochemical Analysis of Cyclooxygenase Expression in Human Skin

   • ShaAvhree Y. Buckman, Alane T. Koki, Dorothy A. Edwards, Alice P. Pentland

   Pages 35-43

6. Separation of Hydroxyeicosatetraenoic Acids and Leukotrienes Originating from Kidney Tissue by C-18 Reverse-Phase High-Pressure Liquid Chromatography

   • Mukut Sharma, Beatrice Hucke, Elias A. Lianos

   Pages 45-54

7. Localization and Distribution of Cyclooxygenase-2 in Brain Tissue by Immunohistochemistry

   • M. Kerry O’Banion, John A. Olschowka

   Pages 55-66

8. Assessment of Cyclooxygenase Protein Expression by Western Blotting

   • Zhonghong Guan, Aubrey R. Morrison

   Pages 67-76

9. Assessment of Arachidonic Acid Distribution into Phospholipids of Inflammatory Cells

   • Alfred N. Fonteh

   Pages 77-89

10. Determination of Phospholipase A2s Expression in Mast Cells by Reverse-Transcriptase Polymerase Chain Reaction

    • Alfred N. Fonteh, William Reed, James M. Samet

    Pages 91-105

11. Enzyme immunoassays (EIA) of Eicosanoids

    • Kingsley Yin, Lloyd J. Forman

    Pages 107-118

12. Transcellular Regulation of Eicosanoid Biosynthesis

    • Karsten Gronert, Clary B. Clish, Mario Romano, Charles N. Serhan

    Pages 119-144

13. Assessment of Cellular Localization of the Thromboxane A2 Receptor by Immunocytochemistry

    • Samuel C. Blackman, Catherine Borg, David C. Yeomans, Guy C. Le Breton

    Pages 145-171

14. Epoxyeicosatrienoic Acids

    • John D. Imig

    Pages 173-192

15. Assessment of the Expression of Prostaglandin Synthase-2 in Swiss 3T3 Fibroblasts

    • Srinivasa T. Reddy, Harvey R. Herschman

    Pages 193-200

16. Assessment of 5-Lipoxygenase Activity and Cellular Distribution

    • Robert A. Lepley

    Pages 201-211

17. Leukotrienes, Lipoxins, and Hydroxyeicosatetraenoic Acids

    • Eric G. Spokas, Joshua Rokach, Patrick Y. -K. Wong

    Pages 213-247

18. Whole Blood Assays for Evaluation of Thromboxane Synthase Inhibition

    • Eric G. Spokas, Nancy S. Nicholson, Osman D. Suleymanov, Gerald M. Walsh, David Tuffin

    Pages 249-256

19. Novel Eicosanoids

    • L. Jackson Roberts II, Cynthia J. Brame, Yan Chen, Jason D. Morrow

    Pages 257-285

20. Back Matter

    Pages 287-296

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In Eicosanoid Protocols, Elias A. Lianos and a panel of hands-on experts present cutting-edge methods for the study of eicosanoids, including prostaglandins, thromboxanes, and leukotrienes. The readily reproducible methods described hereconcentrate on studying the regulation of expression and function of enzymes, particularly cyclooxygenase (and its two isoforms), phospholipase A2, and lipoxygenases involved in the synthesis of established eicosanoids. Additional chapters are devoted to the characterization and distribution of the thromboxane A2 receptor in tissues and the biological roles of novel eicosanoids.
Timely and authoritative, the methods in this book will help their users in exploring the pathobiology of inflammation. Eicosanoid Protocols offers new and established researchers powerful, state-of-the-art tools to probe the regulation and function of eicosanoids.

"...well-produced, timely book..."-The Quarterly Review of Biology

"A very useful book for all specialists on eicosanoid acids, which was lacking until now in the literature." - Cellular and Molecular Biology

• Medical College of Wisconsin, Milwaukee

  Elias A. Lianos

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