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Calcium Signaling Protocols

  • Book
  • © 1999

Overview

Editors:
  1. David G. Lambert

    1. University of Leicester, UK

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Part of the book series: Methods in Molecular Biology (MIMB, volume 114)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xiii
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  2. General

    1. Front Matter

      Pages 1-1
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    2. Fluorescent Measurement of [Ca2+]c

      • Alec W. M. Simpson
      Pages 3-30

    3. Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2

      • Robert A. Hirst, Charlotte Harrison, Kazuyoshi Hirota, David G. Lambert
      Pages 31-39

    4. Measurement of [Ca2+]i in Cell Suspensions Using Indo-1

      • Adriaan Nelemans
      Pages 41-47

  3. Specialist Measurement Systems

    1. Front Matter

      Pages 49-49
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    2. Confocal Microscopy

      • Laura C. Mongan, Joanne Gormally, Andrew R. D. Hubbard, Christopher d’Lacey, Colin D. Ockleford
      Pages 51-74

    3. Measurement of Intracellular Calcium Concentration Using Confocal Microscopy

      • Carmen Perez-Terzic, Marisa Jaconi, Lisa Stehno-Bittel
      Pages 75-91

    4. Single Cell and Subcellular Measurement of Intracellular Ca2+ Concentration ([Ca2+]i)

      • Anthony J. Morgan, Andrew P. Thomas
      Pages 93-123

    5. Measurement of [Ca2+] Using the Fluorometric Imaging Plate Reader (FLIPR)

      • Elaine Sullivan, Emily M. Tucker, Ian L. Dale
      Pages 125-133

  4. Nonelectrophysiological measurement of Ca2+ channel activity

    1. Front Matter

      Pages 135-135
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    2. Measurement of Ca2+ Entry Using 45Ca2+

      • Mercedes Villarroya, Manuela G. López, María F. Cano-Abad, Antonio G. García
      Pages 137-147

    3. Measurement of [3H]PN200-110 and [125I]ω-Conotoxin MVIIA Binding

      • Kazuyoshi Hirota, David G. Lambert
      Pages 149-161

  5. Measurement of Ins(1,4,5)P3 and Ca2+ Release from Intracellular Stores

    1. Front Matter

      Pages 163-163
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    2. Measurement of Inositol (Poly)phosphate Formation Using [3H]Inositol Labeling Protocols in Permeabilized Cells

      • Philip Swigart, Shamshad Cockcroft
      Pages 165-173

    3. Measurement of Inositol(1,4,5)trisphosphate Using a Stereospecific Radioreceptor Mass Assay

      • Darren Smart
      Pages 175-183

    4. Measurement of Calcium Fluxes in Permeabilized Cells Using a 45Ca2+ Uptake and Release Assay

      • Robert A. Wilcox
      Pages 185-192

    5. Microinjection of myo-Inositol(1,4,5)trisphosphate and Other Calcium-Mobilizing Agents into Intact Adherent Cells

      • Robert A. Wilcox, Ian D. Forsythe, Terence J. McCann
      Pages 193-208

    6. Photolysis of Caged Calcium Using a Low-Cost Flash Unit

      • Gommert A. van Koeveringe, Ron van Mastrigt
      Pages 209-220

    7. Measurement of Ca2+ Flux Through Ins(1,4,5)P3 Receptor-Ca2+ Channels in Lipid Bilayers (“Dip-Tip” and “Schindler” Methodology)

      • Edwin C. Thrower
      Pages 221-233
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About this book

2+ The regulation of intracellular Ca is a common theme presented in many 2+ papers over the last 20 or so years, and the description of the Ca -sensitive indicator dye fura 2 in 1985 resulted in a massive increase in these types of 2+ studies. Aspects of the regulation of intracellular Ca have been dealt with in many of the subsequent chapters and will therefore not be covered again. Calcium Signaling Protocols results from a chance discussion with Dr. R. I. Norman of the Department of Medicine at Leicester University and r- resents a major effort from a group of extremely helpful and very patient - thors. Putting a book like this together takes time and I am indebted to these authors without whom this project would have remained a chance discussion. I am also very grateful to Professor J. M. Walker, the series editor, for all his help and advice over the course of this project and particularly his help editing the first batch of chapters. I would also like to thank Dr E. L. Pallett for help and advice regarding interconversion of Mac and Word files and for archiving chapters.

Reviews

". . .recommended to investigators interested in calcium measurement using optical methods. "-Doody's Health Sciences Book Review Journal

"Most protocols identify required materials such as buffers, reagents and cell lines. Methods are presented in a step-by-step format. Hopefully these protocols are adaptable by scientists for use in specific research. Certainly, the methods are specific enough not to leave the researcher stranded. Calcium Signaling Protocols is recommended for academic health and science libraries who support academic research."-E-Streams

Editors and Affiliations

  • University of Leicester, UK

    David G. Lambert

Bibliographic Information

  • Book Title: Calcium Signaling Protocols

  • Editors: David G. Lambert

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592592503

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 1999

  • eBook ISBN: 978-1-59259-250-0Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 359

  • Number of Illustrations: 61 b/w illustrations, 2 illustrations in colour

  • Topics: Biochemistry, general

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