Skip to main content
  • Book
  • © 1999

Vascular Disease

Molecular Biology and Gene Transfer Protocols

Editors:

Part of the book series: Methods in Molecular Medicine (MIMM, volume 30)

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (34 protocols)

  1. Front Matter

    Pages i-xiv
  2. Isolation of Genes Expressed in Vasular Tissue

    1. Front Matter

      Pages 37-37
    2. Efficient Extraction of RNA from Vascular Tissue

      • Catherine F. Townsend, Christopher M. H. Newman, Sheila E. Francis
      Pages 39-46
    3. Preparation of cDNA Libraries from Vascular Cells

      • Mark E. Lieb, Mark B. Taubman
      Pages 47-57
    4. Screening cDNA Libraries Using Partial Probes to Isolate Full-Length cDNAs from Vascular Cells

      • Csilla Csortos, Virginie Lazar, Joe G. N. Garcia
      Pages 59-72
    5. Identification of Novel Protein Kinases in Vascular Cells

      • Ian Zachary, Spiros Servos, Barbara Herren
      Pages 111-129
  3. Methods for Mapping Transcriptional Start Sites and Measurement of Promoter Activity in Vascular Cells

    1. Front Matter

      Pages 131-131
    2. Nuclear Run-On Assay to Study Gene Transcription in Vascular Cells

      • Ulrich Laufs, James K. Liao
      Pages 151-157
  4. Molecular Analysis of MRNA Expression in Vascular Cells

    1. Front Matter

      Pages 169-169

About this book

Molecular biology has revolutionized research into vascular disease. Over the past 20 years molecular techniques have enabled us to both elucidate - lecular mechanisms in vascular disease and identify appropriate therapies. The vast explosion in technical knowledge and the array of protocols that become more advanced and intricate by the day lead us into new and exciting areas of research that were previously unobtainable. Vascular Disease: Molecular Biology and Gene Transfer Protocols - scribes today’s most powerful molecular methods for the investigation of the pathogenesis of vascular disease. The protocols are highly detailed, allowing beginners who have little experience in either vascular biology or molecular biology to embark on new molecular projects. This book is also suited to more experienced molecular biologists who wish to grasp new methods for stu- ing the involvement of genes in normal vascular physiology and in diseased states. It is well established that cardiovascular disease progression has a s- stantial genetic influence. Part I describes three methods that have been used successfully to identify specific mutations in candidate genes involved in c- diovascular disorders. These mutations include both single-stranded conf- mational polymorphism analysis and heteroduplex detection methods. In addition, technology to map new genes to specific regions of chromosomes by high-resolution mapping is described.

Reviews

"...The chapters are clearly written so that complex techniques are reduced to their individual components...this book will be a major asset to a molecular biology laboratory involved in vascular research."-Atherosclerosis

"This collection of very detailed molecular biology protocols was compiled...The first five parts present protocols that are designed to identity and characterize genes in cardiovascular disease, including methods for detecting specific gene mutation, identifying new genes, characterizing the levels and regulation of expression of the new genes, and apoptotic methods. The final two parts contain in vivo and in culture gene transfer protocols used in cardiovascular disease and protocols for clinical gene therapy....an excellent reference for molecular biologists in the field of vascular disease." - Quarterly Review of Biology

"...the authors of the individual chapters have made every effort to relate their particular techniques to the study of vascular tissues....the editor has assembled a broad base of well established authors to describe their protocols....this is a good book....this book may prove worthwhile in helping to ease the load on the typical physician-scientist trying to care for patients and at the same time direct his or her laboratory research program."- Texas Heart Institute Journal

"The book is the result of the contribution of distinguished experts in the field. Each chapter of the book is introduced by a theoretical summary of the goal of the technique with excellent diagrams followed by the material used, the basic procedures and the methods. Critical notes and references end each chapter. This important publication directed to molecular biologists covers the latest aspects of molecular procedures for the study of cardiovascular diseases and confirms, if necessary, the irreversible revolution in cardiovascular research."- Acta Cardiologica

Editors and Affiliations

  • Bristol Heart Institute, University of Bristol, Bristol, UK

    Andrew H. Baker

Bibliographic Information

  • Book Title: Vascular Disease

  • Book Subtitle: Molecular Biology and Gene Transfer Protocols

  • Editors: Andrew H. Baker

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/1592592473

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1999

  • Hardcover ISBN: 978-0-89603-731-1Published: 01 August 1999

  • Softcover ISBN: 978-1-61737-155-4Published: 09 November 2010

  • eBook ISBN: 978-1-59259-247-0Published: 01 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: XV, 441

  • Topics: Vascular Surgery

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access