Gene Probes

1. Front Matter

   Pages i-x

   PDF

2. Target Format and Hybridization Conditions

   • Alex Reid

   Pages 1-11

3. End Labeling Procedures

   • Elena Hilario

   Pages 13-18

4. Photobiotin Labeling

   • Elena Hilario

   Pages 19-22

5. Nick Translation

   • Alex Reid

   Pages 23-25

6. Random Primed Labeling

   • Alex Reid

   Pages 27-28

7. Design and Evaluation of 16S rRNA-Targeted Oligonucleotide Probes for Fluorescence In Situ Hybridization

   • Philip Hugenholtz, Gene W. Tyson, Linda L. Blackall

   Pages 29-42

8. Evaluation of Alterations in the Tumor Suppressor Genes INK4A and INK4B in Human Bladder Tumors

   • Irene Orlow, Carlos Cordon-Cardo

   Pages 43-59

9. The β-Tubulin Gene Region as a Molecular Marker to Distinguish Leishmania Parasites

   • Alexis Mendoza-León, Luis Luis, Clara Martínez

   Pages 61-83

10. Detection of Malignant Plasma Cells in the Bone Marrow and Peripheral Blood of Patients with Multiple Myeloma

    • Ross D. Brown, P. Joy Ho

    Pages 85-91

11. Detection of Chlamydia trachomatis by DNA Amplification

    • Peter Timms, Sarah Mathews

    Pages 93-100

12. Isolation and Identification of Campylobacter Species by Use of Selective Enrichment, Nucleic Acid Amplification Methods, and Gene Probes

    • Alex van Belkum, Nicole van den Braak, Leen-Jan van Doorn, Hubert Endtz

    Pages 101-111

13. Diagnosis of Human Papillomavirus Using In Situ Hybridization and In Situ Polymerase Chain Reaction

    • Gerard J. Nuovo

    Pages 113-136

14. Quantitation of In Situ Hybridization Using Image Analysis of Radioactively Labeled RNA Probes

    • Teresa Bisucci, Tim D. Hewitson, Ian A. Darby

    Pages 137-147

15. Stable Isotope Probing for Detection of Methanotrophs After Enrichment with 13CH4

    • Stefan Radajewski, J. Colin Murrell

    Pages 149-157

16. Detection of Enterococci in Freshwater and Seawater (16S and 23S rRNA Enterococcus Oligonucleotide Probes)

    • Gillian D. Lewis, Sally A. Anderson, Susan J. Turner

    Pages 159-169

17. Analysis of 16S–23S rRNA Intergenic Spacer of Vibrio cholerae and Vibrio mimicus for Detection of These Species

    • Jongsik Chun, Irma N. G. Rivera, Rita R. Colwell

    Pages 171-178

18. Colony Hybridization of Bacterial Isolates with Burkholderia cepacia-Specific Probes

    • Laura G. Leff

    Pages 179-185

19. In Situ Hybridization of Burkholderia cepacia Cells

    • Christopher J. McNamara, Laura G. Leff

    Pages 187-192

20. Detection of Polychlorinated Biphenyl-Degrading Organisms in Soil

    • John Sanseverino, Alice C. Layton, Gary S. Sayler

    Pages 193-209

Senior scientists Marilena Aquino de Muro and Ralph Rapley have brought together an outstanding collection of time-tested protocols for designing and using genes probes in a wide variety of applications. The applications covered range from searching for specific genes in the human chromosome to the detection of microorganisms and their toxins in the environment and in food samples, as well as in the diagnosis of human disease. Helpful tutorials explain the principles of gene probe design, labeling, detection, target formation, and hybridization conditions.

"In this book, the editors have brought together a comprehensive collection of time-tested protocols for designing and using gene probes in a wide variety of applications." -Clinical Laboratory International

• CABI Bioscience UK Centre, Surrey, UK

  Marilena Aquino Muro

• University of Hertfordshire, UK

  Ralph Rapley

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