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SV40 Protocols

  • Book
  • © 2001

Overview

Editors:
  1. Leda Raptis

    1. Queen’s University, Kingston, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 165)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Propagation of Wild-Type and Mutant SV40

    • James D. Tremblay, Kris F. Sachsenmeier, James M. Pipas
    Pages 1-7

  3. Propagation and Assay of the JC Virus

    • Christine K. Liu, Walter J. Atwood
    Pages 9-17

  4. Propagation and Assay of BK Virus

    • Wendy A. Knowles
    Pages 19-31

  5. Molecular Methods for Identification and Genotyping of BK Virus

    • Li Jin
    Pages 33-48

  6. Methods for Studying Interactions Between Simian Virus 40 T-Antigen and the Viral Origin of Replication

    • Kodangattil R. Sreekumar, Brett A. Barbaro, Andrea E. Prack, Peter A. Bullock
    Pages 49-67

  7. Cell-Free Transcription of SV40

    • Richard J. Kraus, Janet E. Mertz
    Pages 69-85

  8. Quantitation and Structural Analysis of SV40 RNAs

    • Richard J. Kraus, Janet E. Mertz
    Pages 87-101

  9. Generation of Recombinant SV40 Vectors for Gene Transfer

    • David S. Strayer, Maria Lamothe, Danlan Wei, Joseph Milano, Rumi Kondo
    Pages 103-117

  10. Production of SV40 Proteins in Insect Cells and In Vitro Packaging of Virions and Pseudovirions

    • Ziv Sandalon, Ariella Oppenheim
    Pages 119-128

  11. Using Retroviral Vectors to Express SV40 Tumor Antigens

    • Van Cherington, Cynthia Higgins
    Pages 129-150

  12. Neoplastic Transformation Assays

    • Leda Raptis, Adina Vultur
    Pages 151-164

  13. Methods to Assess Inhibition of Adipocyte Differentiation by SV40 Large T Antigen

    • Cynthia Higgins, Van Cherington
    Pages 165-183

  14. SV40-Mediated Immortalization

    • Mary Judith Tevethia, Harvey L. Ozer
    Pages 185-199

  15. Biology of p53 and SV40 Large T Association

    • Ximena Montano
    Pages 201-211

  16. Binding of SV40 Large T Antigen to the Retinoblastoma Susceptibility Gene Product and Related Proteins

    • Juan Zalvide, James A. DeCaprio, Hilde Stubdal
    Pages 213-218

  17. In situ Electroporation for the Measurement of c-Ras Activation by SVLT

    • Heather L. Brownell, Leda Raptis
    Pages 219-228

  18. Role of SV40 Small t in Cell Lysis, Transformation, and Signaling

    • Ximena Montano, Kathy Rundell
    Pages 229-242

  19. Cytotoxic T Lymphocytes in SV40 Infections

    • Todd D. Schell, Satvir S. Tevethia
    Pages 243-256

  20. Detection of SV40 DNA Sequences in Human Tissue

    • John A. Lednicky, Robert L. Garcea
    Pages 257-267
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About this book

Simian virus 40 gained notoriety in the 1960s because it was found to be a contaminant of polio and adenovirus vaccines that had been administered to millions of healthy individuals worldwide. The public health implications of this revelation provided the initial impetus for an in-depth study of SV40 biology. Later work showed that SV40 DNA sequences as well as infectious virus are in fact found in human tumors and may have contributed to oncog- esis. It also turned out that SV40 uses mostly cellular machinery to carry out many steps in viral infection, which makes it a powerful probe for examining many fundamental questions in eukaryotic molecular biology. SV40 Pro- cols consolidates a number of well-tested step-by-step techniques in one v- ume; experts with hands-on experience in particular methods give detailed accounts of their optimized experimental protocols, so that the beginner, as well as more experienced researchers, may readily overcome problems of ambiguity often present in the literature. As with other DNA tumor viruses, the response of cultured cells to SV40 infection depends upon the species being infected. Monkey cells s- port virus production, which leads to their death, whereas rodent cells p- duce only the early proteins and acquire a transformed phenotype. Thus, SV40 Protocols is organized in two sections. The first relates to assays of the lytic cycle of the virus, and the second deals with transformation.

Editors and Affiliations

  • Queen’s University, Kingston, Canada

    Leda Raptis

Bibliographic Information

  • Book Title: SV40 Protocols

  • Editors: Leda Raptis

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592591175

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2001

  • Hardcover ISBN: 978-0-89603-653-6Published: 26 December 2000

  • Softcover ISBN: 978-1-4899-4207-4Published: 14 August 2013

  • eBook ISBN: 978-1-59259-117-6Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 310

  • Topics: Microbiology

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