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  • Book
  • © 2001

Matrix Metalloproteinase Protocols

Editors:

  1. Ian M. Clark

    1. University of East Anglia, Norwich, UK

    View editor publications

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Part of the book series: Methods in Molecular Biology (MIMB, volume 151)

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Table of contents (33 protocols)

  1. Front Matter

    Pages i-xv
    PDF

  2. MMPs and TIMPs—An Overview of the Field

    1. Front Matter

      Pages 1-179
      PDF

    2. MMPs and TIMPs

      • J. Frederick Woessner Jr.
      Pages 1-23

    3. Strategies for Cloning New MMPs and TIMPs

      • Gloria Velasco, Carlos López-Otín
      Pages 25-44

    7. Models for Gain-of-Function and Loss-of-Function of MMPs

      • Lisa M. Coussens, Steven D. Shapiro, Paul D. Soloway, Zena Werb
      Pages 149-179

  3. Expression and Purification of MMPs and TIMPs

    1. Front Matter

      Pages 181-304
      PDF

    2. Expression of MMPs andTIMPs in Mammalian Cells

      • Karen M.-L. Yeow, Blaine W. Phillips, P. Paul Beaudry, Kevin J. Leco, Gillian Murphy, Dylan R. Edwards
      Pages 181-189

    3. Expression of Recombinant Matrix Metalloproteinases in Escherichia coli

      • L. Jack Windsor, Darin L. Steele
      Pages 191-205

    6. Expression of Recombinant Membrane-Type MMPs

      • Michael J. Butler, Marie-Pia d’Ortho, Susan J. Atkinson
      Pages 239-255

    9. Purification of MMPs and TIMPs

      • Ken-ichi Shimokawa, Hideaki Nagase
      Pages 275-304

  4. Detection of MMPs and TIMPs

    1. Front Matter

      Pages 305-375
      PDF

    2. Monitoring MMP andTIMP mRNA Expression by RT-PCR

      • Howard Wong, Huong Muzik, Lori L. Groft, Marc A. Lafleur, Charles Matouk, Peter A. Forsyth et al.
      Pages 305-320
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About this book

Research in the matrix metalloproteinase field began with the demonstration by Gross and Lapière, in 1962, that resorbing tadpole tail expressed an enzyme that could degrade collagen gels. These humble beginnings have led us to the elucidation of around twenty distinct vertebrate MMPs, along with a variety of homologs from such diverse organisms as sea urchin, plants, nematode worm, and bacteria. This, coupled with four known specific inhibitors of MMPs, the TIMPs, gives a complex picture. Part I of Matrix Metalloproteinase Protocols provides the reader with a selective overview of the MMP arena, and a chance to come to grips with where the field has been, where it is, and where it is going. I hope that this complements all of the methodology that comes later. Part II presents the reader with a diverse set of methods for the expression and purification of MMPs and TIMPs, bringing together the long and often hard-earned experience of a number of researchers. Part III allows the reader to detect MMPs and TIMPs at both the protein and mRNA level, whereas Part IV gives the ability to assay MMP and TIMP activities in a wide variety of circumstances.
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Reviews

"The editor, Ian M. Clark, has done an excellent job in putting together a collection of articles that span what is now a fairly wide field. ...the content and writing are of a high standard...In chapter 3, covering structural studies and contributed by Wolfram Bode and Klaus Maskos, the authors point out the absence of specific discussions of three-dimensional structures amongst the plethora of general reviews on MMPs and TIMPs. Their contribution expertly fills that void....Indeed the 'Notes' section, at the end of each chapter, is where the benefit of much, often hard-earned, experience is to be reaped and is a valuable addition in almost every case. The last two sections, between them, cover detection of MMp and TIMP expression at both RNA and protein levels, and actively assays. Hore, particular mention must be made of the chapter dealing with zymography and reverse zymography in which Susan Hawkes and coauthors have carefully explained this technique. Their use of figures with detailed interpretation is really excellent and alone could justify the purchase of this book for many labs....this book is recommended as a reference text for all labs engaged in MMP research. There is enough variety for established research labs to find some new methods, and, for newcomers to the field, it should make working with MMPs and their inhibitors a very straightforward prospect." - TRENDS in Cell Biology
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Editors and Affiliations

  • University of East Anglia, Norwich, UK

    Ian M. Clark

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Bibliographic Information

  • Book Title: Matrix Metalloproteinase Protocols

  • Editors: Ian M. Clark

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592590462

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2001

  • eBook ISBN: 978-1-59259-046-9Published: 05 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 545

  • Topics: Biochemistry, general

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eBook USD 74.99
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  • Available as EPUB and PDF
  • Read on any device
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  • Own it forever

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