Overview
- Editors:
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Roberto Bruzzone
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Institut Pasteur, Paris, France
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Christian Giaume
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Collége de France, Paris, France
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Table of contents (25 protocols)
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Tools to Study Connexins
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- Magali Théveniau-Ruissy, Sébastien Alcoléa, Irè Marics, Daniel Gros, Antoon F. M. Moorman, Wouter H. Lamers
Pages 1-32
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- Irene Dunia, Michel Recouvreur, Pierre Nicolas, Nalin M. Kumar, Hans Bloemendal, E. Lucio Benedetti
Pages 33-55
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- Gina E. Sosinsky, Guy A. Perkins
Pages 57-75
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- Mark Yeager, Vinzenz M. Unger
Pages 77-89
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- Judy K. Van Slyke, Linda S. Musil
Pages 117-134
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- Dale W. Laird, Karen Jordan, Qing Shao
Pages 135-142
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- Colin R. Green, Lee yong Law, Jun Sheng Lin, David L. Becker
Pages 175-185
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- Dieter Manthey, Klaus Willecke
Pages 187-199
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Assays For Function
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Front Matter
Pages 201-201
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- I. Martha Skerrett, Mary Merritt, Lan Zhou, Hui Zhu, FengLi Cao, Joseph F. Smith et al.
Pages 225-249
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- Gerhard Dahl, Arnold Pfahnl
Pages 251-268
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- Harold V. M. Van Rijen, Ronald Wilders, Martin B. Rook, Habo J. Jongsma
Pages 269-292
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- Jean Déléze, Bruno Delage, Olfa Hentati-Ksibi, Franck Verrecchia, Jean-Claude Hervé
Pages 313-327
About this book
Direct cell–cell communication is a common property of multicellular organisms that is achieved through membrane channels which are organized in gap junctions. The protein subunits of these intercellular channels, the connexins, form a multigene family that has been investigated in great detail in recent years. It has now become clear that, in different tissues, connexins speak several languages that control specific cellular functions. This progress has been made possible by the availability of new molecular tools and the improvement of basic techniques for the study of membrane channels, as well as by the use of genetic approaches to study protein function in vivo. More important, connexins have gained visibility because mutations in some connexin genes have been found to be linked to human genetic disorders. Connexin Methods and Protocols presents in detail a collection of te- niques currently used to study the cellular and molecular biology of connexins and their physiological properties. The field of gap junctions and connexin research has always been characterized by a multidisciplinary approach c- bining morphology, biochemistry, biophysics, and cellular and molecular biology. This book provides a series of cutting-edge protocols and includes a large spectrum of practical methods that are available to investigate the fu- tion of connexin channels. Connexin Methods and Protocols is divided into three main parts.
Reviews
"This book will be invaluable to those in the connexin field and a fascinating read and useful text to those new to this area of research." -Molecular Biotechnology
Editors and Affiliations
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Institut Pasteur, Paris, France
Roberto Bruzzone
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Collége de France, Paris, France
Christian Giaume
