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Basic DNA and RNA Protocols

  • Book
  • © 1996

Overview

Editors:
  1. Adrian J. Harwood

    1. MRC Laboratory for Molecular Cell Biology, London, UK

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Part of the book series: Methods in Molecular Biology (MIMB, volume 58)

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Table of contents (58 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. DNA Analysis

    1. Front Matter

      Pages 1-1
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    2. The Simultaneous Isolation of RNA and DNA from Tissues and Cultured Cells

      • Frank Merante, Sandeep Raha, Juta K. Reed, Gerald Proteau
      Pages 3-9

    3. Restriction Endonuclease Digestion of DNA

      • Duncan R. Smith
      Pages 11-15

    4. Agarose Gel Electrophoresis

      • Duncan R. Smith
      Pages 17-21

    5. Capillary Blotting of Agarose Gels

      • Duncan R. Smith, David Murphy
      Pages 23-25

    6. Random Primed 32P-Labeling of DNA

      • Duncan R. Smith
      Pages 27-29

    7. Hybridization and Competition Hybridization of Southern Blots

      • Rosemary E. Kelsell
      Pages 31-39

    8. Utilization of DNA Probes with Digoxigenin-Modified Nucleotides in Southern Hybridizations

      • Tim Helentjaris, Tom McCreery
      Pages 41-51

    9. The Preparation of Fluorescein-Labeled Nucleic Acid Probes and Their Detection Using Alkaline Phosphatase-Catalyzed Dioxetane Chemiluminescence

      • Martin Cunningham, Martin Harris
      Pages 53-63

    10. Monitoring Incorporation of Fluorescein into Nucleic Acid Probes Using a Rapid Labeling Assay

      • Martin Cunningham
      Pages 65-66

    11. The Preparation of Horseradish Peroxidase Labeled Nucleic Acid Probes and Their Detection Using Enhanced Chemiluminescence

      • Bronwen Harvey, Ian Durrant, Martin Cunningham
      Pages 67-75

    12. 3′-End Labeling of Oligonucleotides with Fluorescein-11-dUTP and Enhanced Chemiluminescent Detection

      • Martin Cunningham, Ian Durrant, Bronwen Harvey
      Pages 77-82

    13. The Preparation of Riboprobes

      • Dominique Belin
      Pages 83-91

    14. Native Polyacrylamide Gel Electrophoresis

      • Adrian J. Harwood
      Pages 93-96

    15. Use of Silver Staining to Detect Nucleic Acids

      • Lloyd G. Mitchell, Angelika Bodenteich, Carl R. Merril
      Pages 97-103

    16. End-Labeling of DNA Fragments

      • Adrian J. Harwood
      Pages 105-110

  3. RNA Analysis

    1. Northern Blot Analysis

      • Robb Krumlauf
      Pages 113-128

    2. RNA Slot Blotting

      • David Murphy
      Pages 129-130

    3. The RNase Protection Assay

      • Dominique Belin
      Pages 131-136
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Reviews

...this handbook serves its purpose well by being easily readable-FEBS Letters

Editors and Affiliations

  • MRC Laboratory for Molecular Cell Biology, London, UK

    Adrian J. Harwood

Bibliographic Information

  • Book Title: Basic DNA and RNA Protocols

  • Editors: Adrian J. Harwood

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/089603402X

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1996

  • eBook ISBN: 978-1-59259-251-7Published: 09 May 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: 528

  • Topics: Cell Biology

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