Overview
- Editors:
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Gary D. Foster
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Plant Virology Group School of Biological Sciences, University of Bristol, UK
Department of Botany, University of Leicester, UK
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Sally C. Taylor
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Department of Botany, University of Leicester, UK
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Table of contents (55 protocols)
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Introduction
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- Eric D. Miller, Cynthia Hemenway
Pages 25-38
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Virus Isolation, RNA Extraction, and Location of Coat Protein Genes
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- Kenneth E. Palmer, Wendelin H. Schnippenkoetter, Edward P. Rybicki
Pages 41-52
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- Simon N. Covey, Rob J. Noad, Nadia S. Al-Kaff, David S. Turner
Pages 53-63
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- Ichiro Uyeda, Bong-Choon Lee, Yuko Ando, Haruhisa Suga, Yun-Kun He, Masamichi Isogai
Pages 65-75
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- Andrew O. Jackson, John D. O. Wagner
Pages 77-97
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- Diane M. Lawrence, Andrew O. Jackson
Pages 99-106
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- Mounir G. AbouHaidar, Huimin Xu, Kathleen L. Hefferon
Pages 131-143
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- Philip H. Berger, Patrick J. Shiel
Pages 151-160
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- Sylvie German-Retana, Thierry Candresse, Jean Dunez
Pages 161-170
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- Deyin Guo, Edgar Maiss, Günter Adam
Pages 171-181
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About this book
The aim of Plant Virology Protocols is to provide a source of infor- tion to guide the reader through the wide range of methods involved in gen- ating transgenic plants that are resistant to plant viruses. To this end, we have commissioned a wide-ranging list of chapters that will cover the methods required for: plant virus isolation; RNA extraction; cloning coat p- tein genes; introduction of the coat protein gene into the plant genome; and testing transgenic plants for resistance. The book then moves on to treatments of the mechanisms of resistance, the problems encountered with field testing, and key ethical issues surrounding transgenic technology. Although Plant Virology Protocols deals with the cloning and expression of the coat protein gene, the techniques described can be equally applied to other viral genes and nucleotide sequences, many of which have also been shown to afford protection when introduced into plants. The coat protein has, however, been the most widely applied, and as such has been selected to illustrate the techniques involved. Plant Virology Protocols has been divided into six major sections, c- taining 55 chapters in total.