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Capillary Electrophoresis Guidebook

Principles, Operation, and Applications

  • Book
  • © 1996

Overview

Editors:
  1. Kevin D. Altria

    1. Glaxo Research and Development, Ware, Hertfordshire, UK

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Part of the book series: Methods in Molecular Biology (MIMB, volume 52)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-ix
    Download chapter PDF

  2. General Guidelines to the Operation of Capillary Electrophoresis Methods and Instrumentation

    1. Fundamentals of Capillary Electrophoresis Theory

      • Kevin D. Altria
      Pages 3-13

    2. Standard Commercial Instrument Description

      • Kevin D. Altria
      Pages 15-19

    3. Typical Operating Procedures

      • Kevin D. Altria
      Pages 21-28

    4. Method Development/Optimization

      • Kevin D. Altria
      Pages 29-48

    5. Quantitation Procedures

      • Kevin D. Altria
      Pages 49-60

    6. Optimization of Precision in Quantitative Analysis

      • Kevin D. Altria
      Pages 61-70

    7. Optimization of Sensitivity

      • Kevin D. Altria
      Pages 71-81

    8. Method Validation

      • Kevin D. Altria
      Pages 83-97

    9. Fraction Collection

      • Kevin D. Altria
      Pages 99-103

    10. Troubleshooting

      • Kevin D. Altria
      Pages 105-118

    11. Quick Guide to Running a Successful Separation

      • Kevin D. Altria
      Pages 119-122

  3. Applications of Capillary Electrophoresis and Specific Technologies

    1. Micellar Electrokinetic Chromatography

      • Koji Otsuka, Shigeru Terabe
      Pages 125-155

    2. Capillary Gel Electrophoresis

      • András Guttman
      Pages 157-169

    3. Chiral Separations by Capillary Electrophoresis

      • Manus M. Rogan, Kevin D. Altria
      Pages 171-196

    4. Capillary Electrochromatography

      • Iain H. Grant
      Pages 197-209

    5. Application and Limits of Sample Stacking in Capillary Electrophoresis

      • Dean S. Burgi, Ring-Ling Chien
      Pages 211-226

    6. Analysis of Bases, Nucleosides, and (Oligo)nucleotides by Capillary Electrophoresis

      • Herbert E. Schwartz, Kathi J. Ulfelder
      Pages 227-264

    7. Application of Capillary Electrophoresis to Pharmaceutical Analysis

      • Kevin D. Altria
      Pages 265-284

    8. Separation of Peptides and Protein Digests by Capillary Electrophoresis

      • Marzell Herold, Gordon A. Ross, Rudolf Grimm, David N. Heiger
      Pages 285-308
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About this book

This book is intended to be a working guide to the operation of capillary electrophoresis (CE) instrumentation. Since CE is still a rap­ idly maturing technique, detailed validated protocols are not widely established. Therefore, extensive experimental procedures are not pro­ vided for individual analyses. The intention is to provide general guide­ lines on the principles and practice of CE and to give an overview of the specific technologies and important application areas. Part I provides operating instructions for standard commercially available instruments. Guidelines are included for activities such as changing capillaries, method development, quantitative procedures, optimization of precision and sensitivity, and the validation of meth­ ods, fraction collection, and troubleshooting, as well as a quick guide to running a separation. The application range of CE is possibly the most diverse of all analytical techniques and ranges from large, complex macromolecules, such as proteins and nucleic acids, to small solutes, such as organic drugs and inorganic anions and cations.

Reviews

The book is a very useful guidebook for CE practitioners as well as a reference book for researchers and graduate students. I highly recommend this book for libraries and individuals active in the field of CE.-FEBS Letters

Editors and Affiliations

  • Glaxo Research and Development, Ware, Hertfordshire, UK

    Kevin D. Altria

Bibliographic Information

  • Book Title: Capillary Electrophoresis Guidebook

  • Book Subtitle: Principles, Operation, and Applications

  • Editors: Kevin D. Altria

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896033155

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1996

  • Hardcover ISBN: 978-0-89603-315-3Published: 02 November 1995

  • Softcover ISBN: 978-1-61737-011-3Published: 10 November 2010

  • eBook ISBN: 978-1-59259-539-6Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: IX, 349

  • Topics: Analytical Chemistry

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