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Basic Protein and Peptide Protocols

  • Book
  • © 1994

Overview

Editors:
  1. John M. Walker

    1. University of Hertfordshire, Hatfield, UK

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Part of the book series: Methods in Molecular Biology (MIMB, volume 32)

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Table of contents (48 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. The Lowry Method for Protein Quantitation

    • Jaap H. Waterborg, Harry R. Matthews
    Pages 1-4

  3. The Bicinchoninic Acid (BCA) Assay for Protein Quantitation

    • John M. Walker
    Pages 5-8

  4. The Bradford Method for Protein Quantitation

    • Nicholas J. Kruger
    Pages 9-15

  5. Nondenaturing Polyacrylamide Gel Electrophoresis of Proteins

    • John M. Walker
    Pages 17-22

  6. SDS Polyacrylamide Gel Electrophoresis of Proteins

    • Bryan John Smith
    Pages 23-34

  7. Gradient SDS Polyacrylamide Gel Electrophoresis of Proteins

    • John M. Walker
    Pages 35-38

  8. Acetic Acid-Urea Polyacrylamide Gel Electrophoresis of Proteins

    • Bryan John Smith
    Pages 39-47

  9. Electrophoresis of Peptides

    • Ralph C. Judd
    Pages 49-57

  10. Isoelectric Focusing of Proteins in Ultra-Thin Polyacrylamide Gels

    • John M. Walker
    Pages 59-65

  11. The In Vivo Isotopic Labeling of Proteins for Polyacrylamide Gel Electrophoresis

    • Jeffrey W. Pollard
    Pages 67-72

  12. Two-Dimensional Polyacrylamide Gel Electrophoresis of Proteins

    • Jeffrey W. Pollard
    Pages 73-85

  13. Two-Dimensional Polyacrylamide Gel Electrophoresis Using Immobilized pH Gradients in the First Dimension

    • Michael J. Dunn, Joseph M. Corbett
    Pages 87-96

  14. Two-Dimensional Polyacrylamide Gel Electrophoresis Using Flat-Bed Isoelectric Focusing in the First Dimension

    • Michael J. Dunn
    Pages 97-105

  15. Quantification of Proteins on Polyacrylamide Gels (Nonradioactive)

    • Bryan John Smith
    Pages 107-111

  16. Detection of Proteins in Polyacrylamide Gels Using an Ultrasensitive Silver Staining Technique

    • Michael J. Dunn, Samantha J. Crisp
    Pages 113-118

  17. Identification of Glycoproteins on Nitrocellulose Membranes and Gels

    • David J. Thornton, John K. Sheehan, Ingemar Carlstedt
    Pages 119-128

  18. The Release of Oligosaccharides from Glycoproteins

    • Michael J. Davies, Kevin D. Smith, Elizabeth F. Hounsell
    Pages 129-141

  19. Structural Profiling of Oligosaccharides of Glycoproteins

    • Kevin D. Smith, Michael J. Davies, Elizabeth F. Hounsell
    Pages 143-155

  20. Drying Gels

    • Bryan John Smith
    Pages 157-161
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Reviews

...would I buy this volume? I think that the answer to this is yes. It is a useful and concise volume that would be of great value around the lab. I would also recommend it for libraries as it provides an excellent reference source on techniques of protein analysis for undergraduates entering the difficult world of research projects. The value of this volume lies in the fact that any potential reader is liable to use the techniques described in several chapters to pursue a piece of research work. - Trends in Biochemical Sciences

Editors and Affiliations

  • University of Hertfordshire, Hatfield, UK

    John M. Walker

Bibliographic Information

  • Book Title: Basic Protein and Peptide Protocols

  • Editors: John M. Walker

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/089603268X

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1994

  • Hardcover ISBN: 978-0-89603-268-2Published: 26 April 1994

  • Softcover ISBN: 978-0-89603-269-9Published: 26 April 1994

  • eBook ISBN: 978-1-59259-519-8Published: 28 March 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 490

  • Topics: Biochemistry, general

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