Journal of Lipid Research
MethodsA validated LC-MS/MS assay for quantification of 24(S)-hydroxycholesterol in plasma and cerebrospinal fluid
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This work was supported by the Bench-to-Bedside Program, the Office of Rare Diseases Research, the Therapies for Rare and Neglected Diseases (TRND) Program in the National Center for Advancing Translational Sciences of the National Institutes of Health (NIH), and National Institutes of Health Grant 1ZIAHD008824-07 (F.D.P.). Additional support was provided by a grant from Dana's Angels Research Trust (D.S.O). Quantification of 24(S)-HC was performed in the Metabolomics Facility at Washington University (P30 DK020579).
The online version of this article (available at http://www.jlr.org) contains supplementary data in the form of one figure and two tables.
- APCI
atmospheric pressure chemical ionization
- AUC
area under the curve
- CSF
cerebrospinal fluid
- CV
coefficient of variance
- D7-24-HC
25,26,26,26,27,27,27-[2H7]-24(R/S)-hydroxycholesterol
- DQC
dilution quality control
- HP-β-CD
2-hydroxypropyl-β-cyclodextrin
- HQC
high quality control
- LLOQ
lower limit of quantification
- LQC
low quality control
- MQC
middle quality control
- MRM
multiple reaction monitoring
- NIH
National Institutes of Health
- NPC1
Niemann-Pick type C1
- QC
quality control
- RE
relative error
- 24(S)-HC
24(S)-hydroxycholesterol
- ULOQ
upper limit of quantification
Abbreviations