A Novel Sensing Strategy for DNA Analysis Using Nanoscale Graphene Oxide-Coated Microbeads
Graphene oxide (GO) is a powerful material for the detection of various biological targets including nucleic acids, proteins, and metal ions. Herein, we introduce a rapid, sensitive, and selective tool for pathogen detection by combining bead beating-based DNA extraction and graphene
oxide-based fluorescence quenching. This combination was achieved by the fabrication of fluorescent dye-tagged probe/GO/glass beads. The target nucleic acid sequence was extracted from Staphylococcus aureus (S. aureus) using a bead beating tool and GO-coated glass beads (GO beads)
on which an FITC-tagged probe was adsorbed. The interaction between single strand DNA (ss-DNA) and GO was disrupted by the formation of double strand DNA (ds-DNA), resulting in stronger fluorescence emission in ds-DNA-GO mixture compared with ss-DNA-GO mixture. Therefore, when the target DNA
hybridized with the FITC-tagged probes, the probes detached from the GO beads and their fluorescence was renewed. This method allows detection of Staphylococcus aureus at a concentration as low as 105 CFU/ml without sample or signal amplification.
Keywords: Bead Beating Method; Fluorescent Biosensor; Graphene Oxide; Nanoprobe
Document Type: Research Article
Affiliations: School of Integrative Engineering, Chung-Ang University, Heuksuk-dong, Dongjak-gu, Seoul 06974, Republic of Korea
Publication date: 01 November 2017
- Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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