1887

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Volume 61, Issue 3

time-kill studies of antimicrobial agents against blood isolates of imipenem-resistant , including colistin- or tigecycline-resistant isolates Free

Abstract

The emergence of colistin or tigecycline resistance as well as imipenem resistance in poses a great therapeutic challenge. The bactericidal and synergistic effects of several combinations of antimicrobial agents against imipenem-, colistin- or tigecycline-resistant isolates were investigated by time-kill experiments. Six imipenem-resistant blood isolates were examined in this study, including colistin- and tigecycline-susceptible, colistin-resistant but tigecycline-susceptible, and colistin-susceptible but tigecycline-resistant isolates. Time-kill studies were performed using five antimicrobial agents singly or in combinations (imipenem plus colistin, imipenem plus ampicillin-sulbactam, colistin plus rifampicin, colistin plus tigecycline, and tigecycline plus rifampicin) at concentrations of 0.5× and 1× their MICs. Only imipenem was consistently effective as a single agent against all six isolates. Although the effectiveness of combinations of 0.5× MIC antimicrobial agents was inconsistent, combination regimens using 1× MIC of the antimicrobial agents displayed excellent bactericidal activities against all six isolates. Among the combinations of 0.5× MIC antimicrobial agents, the combination of colistin and tigecycline showed synergistic or bactericidal effects against four of the isolates. This time-kill analysis suggests that antimicrobial combinations are effective for killing imipenem-resistant isolates, even if they are simultaneously resistant to either colistin or tigecycline. However, the finding that the combinations of 0.5× MIC antimicrobial agents were effective on only some isolates may warrant further investigation of the doses of combination agents needed to kill resistant .

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© 2012 SGM
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2012-03-01
2024-04-18
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References

  1. Capone A., D’Arezzo S., Visca P., Petrosillo N. 2008; In vitro activity of tigecycline against multidrug-resistant Acinetobacter baumannii. J Antimicrob Chemother 62:422–423 [View Article][PubMed]
     [Google Scholar]
  2. CLSI 2010; Performance Standards for Antimicrobial Susceptibility Testing, 20th informational supplement, M100–S20. Wayne, PA: Clinical and Laboratory Standards Institute;
  3. Dijkshoorn L., Nemec A., Seifert H. 2007; An increasing threat in hospitals: multidrug-resistant Acinetobacter baumannii. Nat Rev Microbiol 5:939–951 [View Article][PubMed]
     [Google Scholar]
  4. Doi Y., Husain S., Potoski B. A., McCurry K. R., Paterson D. L. 2009; Extensively drug-resistant Acinetobacter baumannii. Emerg Infect Dis 15:980–982 [View Article][PubMed]
     [Google Scholar]
  5. Eliopoulos G. M., Moellering R. C. 1996; Antimicrobial combinations. In Antibiotics in Laboratory Medicine, 4th edn. Edited by Lorian V. Baltimore, MA: Williams & Wilkins;
     [Google Scholar]
  6. Falagas M. E., Bliziotis I. A., Siempos I. I. 2006; Attributable mortality of Acinetobacter baumannii infections in critically ill patients: a systematic review of matched cohort and case-control studies. Crit Care 10:R48 [View Article][PubMed]
     [Google Scholar]
  7. Gordon N. C., Png K., Wareham D. W. 2010; Potent synergy and sustained bactericidal activity of a vancomycin-colistin combination versus multidrug-resistant strains of Acinetobacter baumannii. Antimicrob Agents Chemother 54:5316–5322 [View Article][PubMed]
     [Google Scholar]
  8. Hawley J. S., Murray C. K., Jorgensen J. H. 2008; Colistin heteroresistance in Acinetobacter and its association with previous colistin therapy. Antimicrob Agents Chemother 52:351–352 [View Article][PubMed]
     [Google Scholar]
  9. Hornsey M., Wareham D. W. 2011; In vivo efficacy of glycopeptide-colistin combination therapies in a Galleria mellonella model of Acinetobacter baumannii infection. Antimicrob Agents Chemother 55:3534–3537 [View Article][PubMed]
     [Google Scholar]
  10. Kim C. K., Lee Y., Lee H., Woo G. J., Song W., Kim M. N., Lee W. G., Jeong S. H., Lee K., Chong Y. other authors 2010; Prevalence and diversity of carbapenemases among imipenem-nonsusceptible Acinetobacter isolates in Korea: emergence of a novel OXA-182. Diagn Microbiol Infect Dis 68:432–438 [View Article][PubMed]
     [Google Scholar]
  11. Li J., Rayner C. R., Nation R. L., Owen R. J., Spelman D., Tan K. E., Liolios L. 2006a; Heteroresistance to colistin in multidrug-resistant Acinetobacter baumannii. Antimicrob Agents Chemother 50:2946–2950 [View Article][PubMed]
     [Google Scholar]
  12. Li J., Nation R. L., Turnidge J. D., Milne R. W., Coulthard K., Rayner C. R., Paterson D. L. 2006b; Colistin: the re-emerging antibiotic for multidrug-resistant Gram-negative bacterial infections. Lancet Infect Dis 6:589–601 [View Article][PubMed]
     [Google Scholar]
  13. Moland E. S., Craft D. W., Hong S. G., Kim S. Y., Hachmeister L., Sayed S. D., Thomson K. S. 2008; In vitro activity of tigecycline against multidrug-resistant Acinetobacter baumannii and selection of tigecycline-amikacin synergy. Antimicrob Agents Chemother 52:2940–2942 [View Article][PubMed]
     [Google Scholar]
  14. Munoz-Price L. S., Weinstein R. A. 2008; Acinetobacter infection. N Engl J Med 358:1271–1281 [View Article][PubMed]
     [Google Scholar]
  15. Owen R. J., Li J., Nation R. L., Spelman D. 2007; In vitro pharmacodynamics of colistin against Acinetobacter baumannii clinical isolates. J Antimicrob Chemother 59:473–477 [View Article][PubMed]
     [Google Scholar]
  16. Pachón-Ibáñez M. E., Fernández-Cuenca F., Docobo-Pérez F., Pachón J., Pascual A. 2006; Prevention of rifampicin resistance in Acinetobacter baumannii in an experimental pneumonia murine model, using rifampicin associated with imipenem or sulbactam. J Antimicrob Chemother 58:689–692 [View Article][PubMed]
     [Google Scholar]
  17. Pankey G. A., Ashcraft D. S. 2009; The detection of synergy between meropenem and polymyxin B against meropenem-resistant Acinetobacter baumannii using Etest and time-kill assay. Diagn Microbiol Infect Dis 63:228–232 [View Article][PubMed]
     [Google Scholar]
  18. Park Y. K., Jung S. I., Park K. H., Cheong H. S., Peck K. R., Song J. H., Ko K. S. 2009a; Independent emergence of colistin-resistant Acinetobacter spp. isolates from Korea. Diagn Microbiol Infect Dis 64:43–51 [View Article][PubMed]
     [Google Scholar]
  19. Park Y. K., Choi J. Y., Song J. H., Ko K. S. 2009b; In vitro activity of tigecycline against colistin-resistant Acinetobacter spp. isolates from Korea. Int J Antimicrob Agents 33:289–290 [View Article][PubMed]
     [Google Scholar]
  20. Park Y. K., Peck K. R., Cheong H. S., Chung D. R., Song J. H., Ko K. S. 2009c; Extreme drug resistance in Acinetobacter baumannii infections in intensive care units, South Korea. Emerg Infect Dis 15:1325–1327 [View Article][PubMed]
     [Google Scholar]
  21. Park Y. K., Choi J. Y., Jung S. I., Park K. H., Lee H., Jung D. S., Heo S. T., Kim S. W., Chang H. H. et al. 2009d; Two distinct clones of carbapenem-resistant Acinetobacter baumannii isolates from Korean hospitals. Diagn Microbiol Infect Dis 64:389–395 [View Article][PubMed]
     [Google Scholar]
  22. Peleg A. Y., Potoski B. A., Rea R., Adams J., Sethi J., Capitano B., Husain S., Kwak E. J., Bhat S. V., Paterson D. L. 2007; Acinetobacter baumannii bloodstream infection while receiving tigecycline: a cautionary report. J Antimicrob Chemother 59:128–131 [View Article][PubMed]
     [Google Scholar]
  23. Peleg A. Y., Seifert H., Paterson D. L. 2008; Acinetobacter baumannii: emergence of a successful pathogen. Clin Microbiol Rev 21:538–582 [View Article][PubMed]
     [Google Scholar]
  24. Perez F., Hujer A. M., Hujer K. M., Decker B. K., Rather P. N., Bonomo R. A. 2007; Global challenge of multidrug-resistant Acinetobacter baumannii. Antimicrob Agents Chemother 51:3471–3484 [View Article][PubMed]
     [Google Scholar]
  25. Petersen P. J., Labthavikul P., Jones C. H., Bradford P. A. 2006; In vitro antibacterial activities of tigecycline in combination with other antimicrobial agents determined by chequerboard and time-kill kinetic analysis. J Antimicrob Chemother 57:573–576 [View Article][PubMed]
     [Google Scholar]
  26. Scheetz M. H., Qi C., Warren J. R., Postelnick M. J., Zembower T., Obias A., Noskin G. A. 2007; In vitro activities of various antimicrobials alone and in combination with tigecycline against carbapenem-intermediate or -resistant Acinetobacter baumannii. Antimicrob Agents Chemother 51:1621–1626 [View Article][PubMed]
     [Google Scholar]
  27. Song J. Y., Kee S. Y., Hwang I. S., Seo Y. B., Jeong H. W., Kim W. J., Cheong H. J. 2007; In vitro activities of carbapenem/sulbactam combination, colistin, colistin/rifampicin combination and tigecycline against carbapenem-resistant Acinetobacter baumannii. J Antimicrob Chemother 60:317–322 [View Article][PubMed]
     [Google Scholar]
  28. Sopirala M. M., Mangino J. E., Gebreyes W. A., Biller B., Bannerman T., Balada-Llasat J. M., Pancholi P. 2010; Synergy testing by Etest, microdilution checkerboard, and time-kill methods for pan-drug-resistant Acinetobacter baumannii. Antimicrob Agents Chemother 54:4678–4683 [View Article][PubMed]
     [Google Scholar]
  29. Tan T. Y., Ng L. S. Y., Tan E., Huang G. 2007; In vitro effect of minocycline and colistin combinations on imipenem-resistant Acinetobacter baumannii clinical isolates. J Antimicrob Chemother 60:421–423 [View Article][PubMed]
     [Google Scholar]
  30. Tripodi M. F., Durante-Mangoni E., Fortunato R., Utili R., Zarrilli R. 2007; Comparative activities of colistin, rifampicin, imipenem and sulbactam/ampicillin alone or in combination against epidemic multidrug-resistant Acinetobacter baumannii isolates producing OXA-58 carbapenemases. Int J Antimicrob Agents 30:537–540 [View Article][PubMed]
     [Google Scholar]
  31. Vila-Farres X., Garcia de la Maria C., López-Rojas R., Pachón J., Giralt E., Vila J. 2011; In vitro activity of several antimicrobial peptides against colistin-susceptible and colistin-resistant Acinetobacter baumannii. Clin Microbiol Infect http://dx.doi.org/10.1111/j.1469-0691.2011.03581.x (Epub ahead of print) [View Article][PubMed]
     [Google Scholar]
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In vitro time-kill studies of antimicrobial agents against blood isolates of imipenem-resistant Acinetobacter baumannii, including colistin- or tigecycline-resistant isolates
J. Med. Microbiol. 61, 353 (2012); https://doi.org/10.1099/jmm.0.036939-0
/content/journal/jmm/10.1099/jmm.0.036939-0
/content/journal/jmm/10.1099/jmm.0.036939-0
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