Challenging species delimitation in Collembola: cryptic diversity among common springtails unveiled by DNA barcoding
D. Porco A H , A. Bedos B , Penelope Greenslade C , C. Janion D , D. Skarżyński E , M. I. Stevens F , B. Jansen van Vuuren G and L. Deharveng B
+ Author Affiliations
- Author Affiliations
A Université de Rouen – Laboratoire ECODIV, Bâtiment IRESE A, Place Emile Blondel, 76821 Mont Saint Aignan Cedex, France.
B Muséum National d’Histoire Naturelle, UMR7205 «Origine, Structure et Evolution de la Biodiversité», 45 rue Buffon, CP50, 75005 Paris, France.
C Environmental Management, School of Science, Information Technology and Engineering, University of Ballarat, Ballarat, Vic. 3353, Australia.
D Centre for Invasion Biology, Department of Botany and Zoology, Stellenbosch University, Private Bag x1, Matieland 7602, South Africa.
E Zoological Institute, Wrocław University, Przybyszewskiego 63/77, 51-148 Wrocław, Poland.
F South Australian Museum, GPO Box 234, Adelaide, SA 5000, and School of Earth and Environmental Sciences, University of Adelaide, SA 5005, Australia.
G Centre for Invasion Biology, Department of Zoology, University of Johannesburg, PO Box 524, Auckland Park 2006, South Africa.
H Corresponding author: Email: david.porco.gm@gmail.com
Invertebrate Systematics 26(6) 470-477 https://doi.org/10.1071/IS12026
Submitted: 15 April 2012 Accepted: 22 September 2012 Published: 19 December 2012
Abstract
Collembola is one of the major functional groups in soil as well as a model taxon in numerous disciplines. Therefore the accurate identification of specimens is critical, but could be jeopardised by cases of cryptic diversity. Several populations of six well characterised species of springtails were sequenced using the COI barcode fragment as a contribution to the global Collembola barcoding campaign. Each species showed high intraspecific divergence, comparable to interspecific sequence divergence values observed in previous studies and in 10 congeneric species barcoded here as a reference. The nuclear marker, 28S, confirmed all the intraspecific lineages found with COI, supporting the potential specific status of these entities. The implications of this finding for taxonomy and for disciplines relying on species names, such as evolution and ecology, are discussed.
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