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AJP Rep 2015; 05(02): e105-e108
DOI: 10.1055/s-0035-1548726
Case Report
Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

First Pregnancy, Somatic and Psychological Status of a 4-Year-Old Child Born following Annexin V TESA Sperm Separation

Krzysztof Lukaszuk
1   INVICTA Fertility and Reproductive Centre, Gdańsk, Poland
2   INVICTA Fertility and Reproductive Centre, Warsaw, Poland
3   Department of Obstetrics and Gynecological Nursing, Medical University of Gdansk, Gdańsk, Poland
4   Department of Obstetrics and Gynaecology, The Medical Center of Postgraduate Education, Warsaw, Poland
,
Monika Wcislo
2   INVICTA Fertility and Reproductive Centre, Warsaw, Poland
,
Joanna Liss
1   INVICTA Fertility and Reproductive Centre, Gdańsk, Poland
,
Anna Stachowicz
4   Department of Obstetrics and Gynaecology, The Medical Center of Postgraduate Education, Warsaw, Poland
,
Grzegorz Jakiel
4   Department of Obstetrics and Gynaecology, The Medical Center of Postgraduate Education, Warsaw, Poland
,
Mariusz Lukaszuk
1   INVICTA Fertility and Reproductive Centre, Gdańsk, Poland
,
Ewa Pastuszek
1   INVICTA Fertility and Reproductive Centre, Gdańsk, Poland
3   Department of Obstetrics and Gynecological Nursing, Medical University of Gdansk, Gdańsk, Poland
,
Izabela Woclawek-Potocka
5   Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Olsztyn, Poland
,
Antonio Galvao
5   Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Olsztyn, Poland
,
Dorota Bialobrzeska
1   INVICTA Fertility and Reproductive Centre, Gdańsk, Poland
› Author Affiliations
Further Information

Publication History

05 November 2014

12 February 2015

Publication Date:
06 April 2015 (online)

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Abstract

Introduction Sperm DNA integrity is a crucial paternal factor affecting fertilization and pregnancy rates, as well as embryo development.

Case The present case report describes the successful pregnancy after testicular sperm aspiration (TESA) combined with intracytoplasmic sperm injection (ICSI) (TESA-ICSI) in a couple where the male presented high sperm DNA fragmentation. In order to sort damaged sperm presenting DNA fragmentation, magnetic activated cell sorting (MACS) with annexin V microbeads (MACS Miltenyi Biotec, Teterow, Germany) was used.

Conclusion The authors present the first description of a successful medical case using TESA-ICSI annexin V sperm sorting. Additionally, a follow-up of the child at the age of 4 years old was done.

Keywords

sperm - testicular sperm aspiration - intracytoplasmic sperm injection - sperm DNA integrity
 

  • References

  • 1 Lui SC, Weaver SM, Robinson J , et al. A survey of semen donor attitudes. Hum Reprod 1995; 10 (1) 234-238
    CrossrefPubMedGoogle Scholar
  • 2 Shukla KK, Mahdi AA, Rajender S. Apoptosis, spermatogenesis and male infertility. Front Biosci (Elite Ed) 2012; 4: 746-754
    PubMedGoogle Scholar
  • 3 Agarwal A, Said TM. Role of sperm chromatin abnormalities and DNA damage in male infertility. Hum Reprod Update 2003; 9 (4) 331-345
    CrossrefPubMedGoogle Scholar
  • 4 Sakkas D, Alvarez JG. Sperm DNA fragmentation: mechanisms of origin, impact on reproductive outcome, and analysis. Fertil Steril 2010; 93 (4) 1027-1036
    CrossrefPubMedGoogle Scholar
  • 5 Kefer JC, Agarwal A, Sabanegh E. Role of antioxidants in the treatment of male infertility. Int J Urol 2009; 16 (5) 449-457
    CrossrefPubMedGoogle Scholar
  • 6 Sikka SC. Role of oxidative stress and antioxidants in andrology and assisted reproductive technology. J Androl 2004; 25 (1) 5-18
    CrossrefPubMedGoogle Scholar
  • 7 Janny L, Menezo YJR. Evidence for a strong paternal effect on human preimplantation embryo development and blastocyst formation. Mol Reprod Dev 1994; 38 (1) 36-42
    CrossrefPubMedGoogle Scholar
  • 8 Ward WS. Function of sperm chromatin structural elements in fertilization and development. Mol Hum Reprod 2010; 16 (1) 30-36
    CrossrefPubMedGoogle Scholar
  • 9 Bakos HW, Thompson JG, Feil D, Lane M. Sperm DNA damage is associated with assisted reproductive technology pregnancy. Int J Androl 2008; 31 (5) 518-526
    CrossrefPubMedGoogle Scholar
  • 10 Robinson L, Gallos ID, Conner SJ , et al. The effect of sperm DNA fragmentation on miscarriage rates: a systematic review and meta-analysis. Hum Reprod 2012; 27 (10) 2908-2917
    CrossrefPubMedGoogle Scholar
  • 11 Simon L, Brunborg G, Stevenson M, Lutton D, McManus J, Lewis SEM. Clinical significance of sperm DNA damage in assisted reproduction outcome. Hum Reprod 2010; 25 (7) 1594-1608
    CrossrefPubMedGoogle Scholar
  • 12 Zhang L, Wang L, Zhang X , et al. Sperm chromatin integrity may predict future fertility for unexplained recurrent spontaneous abortion patients. Int J Androl 2012; 35 (5) 752-757
    CrossrefPubMedGoogle Scholar
  • 13 Zini A, Boman JM, Belzile E, Ciampi A. Sperm DNA damage is associated with an increased risk of pregnancy loss after IVF and ICSI: systematic review and meta-analysis. Hum Reprod 2008; 23 (12) 2663-2668
    CrossrefPubMedGoogle Scholar
  • 14 Muratori M, Maggi M, Spinelli S, Filimberti E, Forti G, Baldi E. Spontaneous DNA fragmentation in swim-up selected human spermatozoa during long term incubation. J Androl 2003; 24 (2) 253-262
    CrossrefPubMedGoogle Scholar
  • 15 Agarwal A, Nallella KP, Allamaneni SSR, Said TM. Role of antioxidants in treatment of male infertility: an overview of the literature. Reprod Biomed Online 2004; 8 (6) 616-627
    CrossrefPubMedGoogle Scholar
  • 16 Shafik A, Shafik AA, Shafik I, El Sibai O. Sperm DNA fragmentation. Arch Androl 2006; 52 (3) 197-208
    CrossrefPubMedGoogle Scholar
  • 17 Glander HJ, Schaller J. Binding of annexin V to plasma membranes of human spermatozoa: a rapid assay for detection of membrane changes after cryostorage. Mol Hum Reprod 1999; 5 (2) 109-115
    CrossrefPubMedGoogle Scholar
  • 18 Nasr-Esfahani MH, Deemeh MR, Tavalaee M. New era in sperm selection for ICSI. Int J Androl 2012; 35 (4) 475-484
    CrossrefPubMedGoogle Scholar
  • 19 Rawe VY, Boudri HU, Alvarez Sedó C, Carro M, Papier S, Nodar F. Healthy baby born after reduction of sperm DNA fragmentation using cell sorting before ICSI. Reprod Biomed Online 2010; 20 (3) 320-323
    CrossrefPubMedGoogle Scholar
  • 20 Said T, Agarwal A, Grunewald S , et al. Selection of nonapoptotic spermatozoa as a new tool for enhancing assisted reproduction outcomes: an in vitro model. Biol Reprod 2006; 74 (3) 530-537
    CrossrefPubMedGoogle Scholar
  • 21 Lukaszuk K, Liss J, Kunicki M , et al. Anti-Müllerian hormone (AMH) is a strong predictor of live birth in women undergoing assisted reproductive technology. Reprod Biol 2014; 14 (3) 176-181
    CrossrefPubMedGoogle Scholar
  • 22 Lukaszuk K, Liss J, Lukaszuk M, Maj B. Optimization of estradiol supplementation during the luteal phase improves the pregnancy rate in women undergoing in vitro fertilization-embryo transfer cycles. Fertil Steril 2005; 83 (5) 1372-1376
    CrossrefPubMedGoogle Scholar
  • 23 Jaworowska AMA, Szustrowa T. Leiter International Performance Scale P-93. Polish 1993 Standardisation. Psychological Test Laboratory Polish Psychological Association, Warsaw; 1993
    PubMedGoogle Scholar
  • 24 Bee H. Psychologia rozwoju człowieka. 2004. Zysk i S-ka, Poznań
    PubMedGoogle Scholar
  • 25 Vasta R, Haith MM, Miller SA. Psychologia dziecka. 2004. WSiP, Warsaw
    PubMedGoogle Scholar
  • 26 Schaffer RH. Psychologia dziecka. 2011. WN PWN, Warsaw
    PubMedGoogle Scholar
  • 27 Brazelton TB, Sparrow JD. Rozwój dziecka. Od 3 do 6 lat. 2013. GWP, Sopot
    PubMedGoogle Scholar
  • 28 Oleszczuk K, Augustinsson L, Bayat N, Giwercman A, Bungum M. Prevalence of high DNA fragmentation index in male partners of unexplained infertile couples. Andrology 2013; 1 (3) 357-360
    CrossrefPubMedGoogle Scholar
  • 29 Polak de Fried E, Denaday F. Single and twin ongoing pregnancies in two cases of previous ART failure after ICSI performed with sperm sorted using annexin V microbeads. Fertil Steril 2010; 94 (1) 351.e15-351.e18
    PubMedGoogle Scholar